AP2922b

antibody from Abgent

Targeting: PDIA3 ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2922b

Provider

Abgent

Proper citation

Abgent Cat#AP2922b, RRID:AB_1967767

Product name

PDIA3 Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of PDIA3 Antibody (C-term) (Cat. #AP2922b) in A2058 cell line lysates (35ug/lane). PDIA3 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of PDIA3 (arrow) using rabbit polyclonal PDIA3 Antibody (C-term) (Cat. #AP2922b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDIA3 gene (Lane 2) .

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PDIA3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

PDIA3 Antibody (C-term) (Cat. #AP2922b) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50