Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- 41-9898-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 19 Monoclonal Antibody (BA17), eFluor™ 570, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This BA17 monoclonal antibody reacts with human cytokeratin 19 (CK19), a 44-kDa type I (acidic) intermediate filament protein that lacks the non-alpha-helical tail domain present in other keratins. Cytokeratins form the intracellular cytoskeletal network that maintains the integrity and stability of cells and tissues. Cytokeratin 19 is expressed in a wide variety of simple and stratified epithelial tissue. Moreover, cytokeratin 19 expression can be induced by vitamin A, SV40 transformation, and cancer. A soluble form of cytokeratin 19 generated by caspase 3 cleavage has also been found to be secreted by cancer cells, thus possibly indicating tumor metastasis. Cytokeratin 19 often exists as a heterodimer with cytokeratin 7, a type II keratin. Applications Reported: This BA17 antibody has been reported for use in immunohistochemical staining and immunocytochemistry. Applications Tested: This BA17 antibody has been tested by immunocytochemistry on fixed and permeabilized MCF7 cells and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nm
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BA17
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunocytochemistry on fixed and permeabilized MCF7 cells using 20 µg/mL Anti-Human Cytokeratin 19 eFluor® 570. Nuclei are stained with DAPI.