Explore
Validate
Learn
Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA1-46089 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRF1 Monoclonal Antibody (3H11)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Suggested positive control: antigen standard for TERF1 (transient overexpression lysate).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3H11
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Molecular Consequences of Depression Treatment: A Potential In Vitro Mechanism for Antidepressants-Induced Reprotoxic Side Effects.
Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes.
Long-term culture with lipopolysaccharide induces dose-dependent cytostatic and cytotoxic effects in THP-1 monocytes.
Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
Compartmentation of alpha-internexin and neurofilament triplet proteins in cultured hippocampal neurons.
Sołek P, Mytych J, Tabęcka-Łonczyńska A, Koziorowski M
International journal of molecular sciences 2021 Nov 1;22(21)
International journal of molecular sciences 2021 Nov 1;22(21)
Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes.
Mytych J, Wos I, Solek P, Koziorowski M
Experimental cell research 2017 Jan 15;350(2):358-367
Experimental cell research 2017 Jan 15;350(2):358-367
Long-term culture with lipopolysaccharide induces dose-dependent cytostatic and cytotoxic effects in THP-1 monocytes.
Mytych J, Romerowicz-Misielak M, Koziorowski M
Toxicology in vitro : an international journal published in association with BIBRA 2017 Aug;42:1-9
Toxicology in vitro : an international journal published in association with BIBRA 2017 Aug;42:1-9
Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
Shaw G, Morse S, Ararat M, Graham FL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2002 Jun;16(8):869-71
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2002 Jun;16(8):869-71
Compartmentation of alpha-internexin and neurofilament triplet proteins in cultured hippocampal neurons.
Benson DL, Mandell JW, Shaw G, Banker G
Journal of neurocytology 1996 Mar;25(3):181-96
Journal of neurocytology 1996 Mar;25(3):181-96
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Toxic concentrations of antidepressants promote DNA fragmentation of treated cells. GC-1 spg and GC-2 spd cells were treated with antidepressants for 48 and 96 h. The TUNEL labeling system to detect the DNA damage was then applied ( A , C ) and the level of TRF1, TRF2 proteins were controlled by Western blot technique. Representative blots ( B , D ) and photos of TUNEL cells are shown (magnification 10x). Blue fluorescence (DAPI) live cells, green fluorescence (FITC) apoptotic cells. Statistical differences were determined using one-way analysis of variance (ANOVA) with Dunnett's post-hoc test; p values < 0.05 were considered statistically significant. Asterisks (*) indicate the comparison between control and antidepressants-treated cells, whereas carets (^) indicate the comparison between the same drugs in different periods (48 vs. 96). Bars indicate mean +- SD, n = 3, ***/^^^ p < 0.001, **/^^ p < 0.01, */^ p < 0.05, no indication-no statistical significance.
