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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [7]
- Immunohistochemistry [1]
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- Product number
- PA5-85462 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAFF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 1 mg/mL
Submitted references Deepening the Whole Transcriptomics of Bovine Liver Cells Exposed to AFB1: A Spotlight on Toll-like Receptor 2.
Iori S, Pauletto M, Bassan I, Bonsembiante F, Gelain ME, Bardhi A, Barbarossa A, Zaghini A, Dacasto M, Giantin M
Toxins 2022 Jul 20;14(7)
Toxins 2022 Jul 20;14(7)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAFF in A) Huh7 whole cell lysate, B) HepG2 whole cell lysate using MAFF polyclonal antibody (Product # PA5-85462) using 30 µg of sample at a dilution of 1:2000. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAFF Polyclonal Antibody detects MAFF protein by Western blot analysis. A. 30 µg Huh7 whole cell lysate/extract. B. 30 µg HepG2 whole cell lysate/extract.12 % SDS-PAGE. MAFF Polyclonal Antibody (Product # PA5-85462) dilution: 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAFF in non-transfected (-) and MAFF -transfected (+, ) 293T whole cell extracts using MAFF polyclonal antibody (Product # PA5-85462) using 30 µg of sample at a dilution of 1:20000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MAFF was performed by separating 30 µg of A431 whole cell and nuclear extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a MAFF Polyclonal Antibody (Product # PA5-85462) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAFF Polyclonal Antibody detects MAFF protein by Western blot analysis. A. 30 µg Huh7 whole cell lysate/extract. B. 30 µg HepG2 whole cell lysate/extract.12 % SDS-PAGE. MAFF Polyclonal Antibody (Product # PA5-85462) dilution: 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAFF was performed by separating 50 µg of rat tissue extract by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a MAFF Polyclonal Antibody (Product # PA5-85462) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAFF Polyclonal Antibody detects MAFF protein by western blot analysis. Non-transfected (-) and MAFF -transfected (+, ) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAFF Polyclonal Antibody (Product # PA5-85462) diluted by 1:20,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAFF Polyclonal Antibody detects MAFF protein at cytoplasm and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MAFF Polyclonal Antibody (Product # PA5-85462) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
