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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [7]
- Other assay [1]
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- Product number
- UM500047 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM500047, RRID:AB_2629061
- Product name
- SOX5 mouse monoclonal antibody,clone UMAB53
- Antibody type
- Monoclonal
- Description
- SOX5 mouse monoclonal antibody,clone UMAB53
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SOX5
- Isotype
- IgG
- Antibody clone number
- UMAB53
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human testicular cancer tissue using anti-SOX5 mouse monoclonal antibody. (UM500047; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human melanoma using anti-SOX5 clone UMAB53 mouse monoclonal antibody (UM500047) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong nuclear and weak cytoplasmic staining is seen in the tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human colon using anti-SOX5 clone UMAB53 mouse monoclonal antibody (UM500047) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Weak cytoplasmic staining is seen in the epithelial cells of the colon.
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human kidney using anti-SOX5 clone UMAB53 mouse monoclonal antibody (UM500047) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Weak cytoplasmic staining is seen in the tubule epithelial cells of the kidney.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human pacreatic cancer using anti-SOX5 clone UMAB53 mouse monoclonal antibody (UM500047) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human thyroid cancer using anti-SOX5 clone UMAB53 mouse monoclonal antibody (UM500047) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic staining is seen in the tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human skin using anti-SOX5 clone UMAB53 mouse monoclonal antibody (UM500047) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong nuclear and cytoplasmic staining is seen in the epidermal cells.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SOX5 mouse monoclonal antibody (UM500047). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
- Validation comment
- 10K-CHIP
