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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF5286 - Provider product page
- Provider
- R&D Systems
- Product name
- Human SOX5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human SOX5 in Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P35711- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human SOX5 by Western Blot. Western blot shows lysates of human lung tissue and exponentially growing LNCaP human prostate cancer cell line whole cell extract. PVDF membrane was probed with 1 µg/mL of Human SOX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5286) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOX5 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
