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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA5-65593 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CREB5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PAAMSNGNMNT MGHMMEMMGS RQDQTPHHHM HSHPHQHQTL PPHHPYPHQH QHPAHHPHPQ PHHQQNH
- Concentration
- 0.06 mg/mL
Submitted references Creb5 establishes the competence for Prg4 expression in articular cartilage.
Zhang CH, Gao Y, Jadhav U, Hung HH, Holton KM, Grodzinsky AJ, Shivdasani RA, Lassar AB
Communications biology 2021 Mar 12;4(1):332
Communications biology 2021 Mar 12;4(1):332
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of CREB5 in human cell line HEK 293 shows localization to nucleus and plasma membrane. Samples were probed using a CREB5 Polyclonal Antibody (Product # PA5-65593).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 The transcription factor Creb5 is differentially expressed in superficial versus deep zone articular chondrocytes and is specifically expressed in Prg4 + cells in synovial joints. a RT-qPCR analysis of Prg4 and Creb5 expression in either SZCs or DZCs cultured in either the absence (white) or presence (gray) of TGF-beta2 (20 ng/ml) for 3 days. Gene expression was assayed by RT-qPCR and normalized to Gapdh . Error bar indicates standard error of the mean ( n = 2 technical repeats). Similar results have been obtained in three independent biological repeats. b Western analysis of proteins in SZCs and DZCs. Similar results have been obtained in two independent biological repeats. c Immunofluorescent staining for CREB5, lubricin, and DAPI (to visualize nuclei) in adult human femoral head articular cartilage. Scale bar equals 50 microns. d Schematic of Prg4 expression in a developing long bone cartilage element. e Expression of Collagen 2a1 (Col2), Prg4, Creb5 , and Matrilin1 (Matn1) in the elbows of P0 mice, as detected by fluorescent in situ hybridization. f Immunofluorescent staining for Creb5 protein and DAPI (to visualize nuclei) in the knee joint of a P0 mouse. In ( e , f ), the Creb5-expressing articular perichondrium is designated by a yellow arrow, the metaphyseal perichondrium is designated by a white arrow; and the scale bars equal 100 microns.
