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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-21446 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR2B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, BCL-1.
- Concentration
- 0.32 mg/mL
Submitted references Loss of ASXL1 in the bone marrow niche dysregulates hematopoietic stem and progenitor cell fates.
Zhang P, Chen Z, Li R, Guo Y, Shi H, Bai J, Yang H, Sheng M, Li Z, Li Z, Li J, Chen S, Yuan W, Cheng T, Xu M, Zhou Y, Yang FC
Cell discovery 2018;4:4
Cell discovery 2018;4:4
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using POLR2B Polyclonal Antibody (Product # PA5-21446). Sample (30 µg of whole cell lysate). Lane A: H1299. 5% SDS PAGE. POLR2B Polyclonal Antibody (Product # PA5-21446) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using POLR2B Polyclonal Antibody (Product # PA5-21446). Sample (30 µg of whole cell lysate). Lane A: H1299. 5% SDS PAGE. POLR2B Polyclonal Antibody (Product # PA5-21446) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using POLR2B Polyclonal Antibody (Product # PA5-21446). Sample (30 µg of whole cell lysate). Lane A: BCL-1. 5% SDS PAGE. POLR2B Polyclonal Antibody (Product # PA5-21446) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of POLR2B in paraformaldehyde-fixed HeLa cells using a POLR2B polyclonal antibody (Product # PA5-21446) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPB2 antibody (Product # PA5-21446) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of POLR2B was performed in paraffin-embedded human breast carcinoma tissue using POLR2B Polyclonal Antibody (Product # PA5-21446) at a dilution of 1:250.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using RPB2 (Product # PA5-21446) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- POLR2B Polyclonal Antibody detects RPB2 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse tongue. RPB2 stained by POLR2B Polyclonal Antibody (Product # PA5-21446) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
