693902

antibody from BioLegend

Targeting: ATF4 CREB-2, TAXREB67, TXREB

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

693902

Provider

BioLegend

Proper citation

BioLegend Cat#693902, RRID:AB_2650720

Product name

Purified anti-ATF4

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W16016A

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (15 µg protein) from non-treated and Tunicamycin (Tm) treated (2 µg/mL, size hours) HeLa and 293T were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-ATF4 (clone W16016A) antibody or competitor’s antibody used at the manufacturers recommended concentration. Proteins were visualized using an HRP goat anti-rat-IgG secondary antibody (clone Poly4054) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.

Conjugate

Unconjugated