Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00000468-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000468-M01, RRID:AB_536980
- Product name
- Mouse Monoclonal ATF4 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ATF4 - activating transcription factor 4 (tax-responsive enhancer element B67)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Polydatin, Natural Precursor of Resveratrol, Promotes Osteogenic Differentiation of Mesenchymal Stem Cells.
Decreased vitamin B12 availability induces ER stress through impaired SIRT1-deacetylation of HSF1.
miR-214 targets ATF4 to inhibit bone formation.
A protective role of heme-regulated eIF2α kinase in cadmium-induced toxicity in erythroid cells.
Heme-regulated eIF2α kinase activated Atf4 signaling pathway in oxidative stress and erythropoiesis.
12/15-Lipoxygenase signaling in the endoplasmic reticulum stress response.
Calcium channel blocker verapamil enhances endoplasmic reticulum stress and cell death induced by proteasome inhibition in myeloma cells.
Calcium channel blocker verapamil enhances endoplasmic reticulum stress and cell death induced by proteasome inhibition in myeloma cells.
Di Benedetto A, Posa F, De Maria S, Ravagnan G, Ballini A, Porro C, Trotta T, Grano M, Muzio LL, Mori G
International journal of medical sciences 2018;15(9):944-952
International journal of medical sciences 2018;15(9):944-952
Decreased vitamin B12 availability induces ER stress through impaired SIRT1-deacetylation of HSF1.
Ghemrawi R, Pooya S, Lorentz S, Gauchotte G, Arnold C, Gueant JL, Battaglia-Hsu SF
Cell death & disease 2013 Mar 21;4:e553
Cell death & disease 2013 Mar 21;4:e553
miR-214 targets ATF4 to inhibit bone formation.
Wang X, Guo B, Li Q, Peng J, Yang Z, Wang A, Li D, Hou Z, Lv K, Kan G, Cao H, Wu H, Song J, Pan X, Sun Q, Ling S, Li Y, Zhu M, Zhang P, Peng S, Xie X, Tang T, Hong A, Bian Z, Bai Y, Lu A, Li Y, He F, Zhang G, Li Y
Nature medicine 2013 Jan;19(1):93-100
Nature medicine 2013 Jan;19(1):93-100
A protective role of heme-regulated eIF2α kinase in cadmium-induced toxicity in erythroid cells.
Wang L, Wang X, Zhang S, Qu G, Liu S
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2013 Dec;62:880-91
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2013 Dec;62:880-91
Heme-regulated eIF2α kinase activated Atf4 signaling pathway in oxidative stress and erythropoiesis.
Suragani RN, Zachariah RS, Velazquez JG, Liu S, Sun CW, Townes TM, Chen JJ
Blood 2012 May 31;119(22):5276-84
Blood 2012 May 31;119(22):5276-84
12/15-Lipoxygenase signaling in the endoplasmic reticulum stress response.
Cole BK, Kuhn NS, Green-Mitchell SM, Leone KA, Raab RM, Nadler JL, Chakrabarti SK
American journal of physiology. Endocrinology and metabolism 2012 Mar 15;302(6):E654-65
American journal of physiology. Endocrinology and metabolism 2012 Mar 15;302(6):E654-65
Calcium channel blocker verapamil enhances endoplasmic reticulum stress and cell death induced by proteasome inhibition in myeloma cells.
Meister S, Frey B, Lang VR, Gaipl US, Schett G, Schlötzer-Schrehardt U, Voll RE
Neoplasia (New York, N.Y.) 2010 Jul;12(7):550-61
Neoplasia (New York, N.Y.) 2010 Jul;12(7):550-61
Calcium channel blocker verapamil enhances endoplasmic reticulum stress and cell death induced by proteasome inhibition in myeloma cells.
Meister S, Frey B, Lang VR, Gaipl US, Schett G, Schlötzer-Schrehardt U, Voll RE
Neoplasia (New York, N.Y.) 2010 Jul;12(7):550-61
Neoplasia (New York, N.Y.) 2010 Jul;12(7):550-61
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATF4 Antibody (2B3) [H00000468-M01] - Western Blot analysis of ATF4 expression in NIH/3T3 ( Cat # L018V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATF4 Antibody (2B3) [H00000468-M01] - Analysis of ATF4 expression in transfected 293T cell line by ATF4 monoclonal antibody (M01), clone 2B3.Lane 1: ATF4 transfected lysate(38.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: ATF4 Antibody (2B3) [H00000468-M01] - Detection limit for recombinant GST tagged ATF4 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ATF4 Antibody (2B3) [H00000468-M01] - Analysis of monoclonal antibody to ATF4 on HeLa cell. Antibody concentration 10 ug/ml.
