AM05643PU-N

antibody from Acris Antibodies GmbH

Targeting: IRF3

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM05643PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM05643PU-N, RRID:AB_1618514

Product name

anti IRF3

Antibody type

Monoclonal

Antigen

Recombinant human IRF3 (108-166 aa) purified from E.coli

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3F10

Vial size

0.1 mg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis on HeLa cells: Cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IRF-3 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IRF-3 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence of human HeLa cells stained with IRF-3 (1:500) with Texas-Red and Hoechst 33342 (Blue) for nucleus staining.