AF521

antibody from Novus Biologicals

Targeting: IL18 IGIF, IL-18, IL-1g, IL1F4

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF521

Provider

Novus Biologicals

Product name

Goat Polyclonal IL-18/IL-1F4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects rat IL-18/IL-1F4 in direct ELISAs and Western blots. In Western blots, approximately 35% cross-reactivity with recombinant mouse IL-18 is observed and approximately 10% cross-reactivity with recombinant human IL-18 is observed.

Reactivity

Rat

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Interleukin-18 and its receptor are expressed in gonadotropin-releasing hormone neurons of mouse and rat forebrain.

Kuwahara-Otani S, Maeda S, Kobayashi K, Minato Y, Tanaka K, Yamanishi K, Hata M, Li W, Hayakawa T, Noguchi K, Okamura H, Yagi H
Neuroscience letters 2017 May 22;650:33-37

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Interleukin-18-mediated microglia/astrocyte interaction in the spinal cord enhances neuropathic pain processing after nerve injury.

Miyoshi K, Obata K, Kondo T, Okamura H, Noguchi K
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Nov 26;28(48):12775-87

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Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.

Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK 3rd, Woods JM, Koch AE
Arthritis and rheumatism 2006 Aug;54(8):2402-14

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Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain.

Felderhoff-Mueser U, Sifringer M, Polley O, Dzietko M, Leineweber B, Mahler L, Baier M, Bittigau P, Obladen M, Ikonomidou C, Bührer C
Annals of neurology 2005 Jan;57(1):50-9

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Role of IL-18 in acute lung inflammation.

Jordan JA, Guo RF, Yun EC, Sarma V, Warner RL, Crouch LD, Senaldi G, Ulich TR, Ward PA
Journal of immunology (Baltimore, Md. : 1950) 2001 Dec 15;167(12):7060-8

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Role of IL-18 in acute lung inflammation.

Jordan JA, Guo RF, Yun EC, Sarma V, Warner RL, Crouch LD, Senaldi G, Ulich TR, Ward PA
Journal of immunology (Baltimore, Md. : 1950) 2001 Dec 15;167(12):7060-8

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

IFN-gamma Secretion Induced by IL-18/IL-1F4 and Neutralization by Rat IL-18/IL-1F4 Antibody. In the presence of Recombinant Mouse IL-12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Rat IL-18/IL-1F4 (Catalog # 521-RL) stimulates IFN-gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN-gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN-gamma secretion elicited by Recombinant Rat IL-18/IL-1F4 (15 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL-18/IL-1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF521). The ND₅₀ is typically 1-3 µg/mL.