210-401-323S

antibody from Rockland Immunochemicals, Inc.

Targeting: IL18 IGIF, IL-18, IL-1g, IL1F4

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

210-401-323S

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#210-401-323S, RRID:AB_11181154

Product name

Anti-Mouse IL-18 (RABBIT) Antibody - 210-401-323S

Antibody type

Polyclonal

Vial size

25 µl

References

Submitted references

Epithelial-derived gasdermin D mediates nonlytic IL-1β release during experimental colitis.

Bulek K, Zhao J, Liao Y, Rana N, Corridoni D, Antanaviciute A, Chen X, Wang H, Qian W, Miller-Little WA, Swaidani S, Tang F, Willard BB, McCrae K, Kang Z, Dubyak GR, Cominelli F, Simmons A, Pizarro TT, Li X
The Journal of clinical investigation 2020 Aug 3;130(8):4218-4234

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A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.

Quach C, Song Y, Guo H, Li S, Maazi H, Fung M, Sands N, O'Connell D, Restrepo-Vassalli S, Chai B, Nemecio D, Punj V, Akbari O, Idos GE, Mumenthaler SM, Wu N, Martin SE, Hagiya A, Hicks J, Cui H, Liang C
Nature communications 2019 Dec 12;10(1):5681

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Extracellular TDP-43 aggregates target MAPK/MAK/MRK overlapping kinase (MOK) and trigger caspase-3/IL-18 signaling in microglia.

Leal-Lasarte MM, Franco JM, Labrador-Garrido A, Pozo D, Roodveldt C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Jul;31(7):2797-2816

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Insulin sensitizers prevent fine particulate matter-induced vascular insulin resistance and changes in endothelial progenitor cell homeostasis.

Haberzettl P, McCracken JP, Bhatnagar A, Conklin DJ
American journal of physiology. Heart and circulatory physiology 2016 Jun 1;310(11):H1423-38

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TRAF3IP2 mediates aldosterone/salt-induced cardiac hypertrophy and fibrosis.

Sakamuri SS, Valente AJ, Siddesha JM, Delafontaine P, Siebenlist U, Gardner JD, Bysani C
Molecular and cellular endocrinology 2016 Jul 5;429:84-92

Immunocytochemistry

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Immunofluorescence microscopy of IL-18 in mouse colon sections. The transversing portion of the large intestine from DSS-exposed (Panel A) and -unexposed mice (Panel B) was excised, rinsed in PBS, and frozen on isopentane cooled with liquid nitrogen. Frozen sections (5 µm) were cut on a Leica CM 1850 cryostat. The slides were fixed for 10 min in 4% paraformaldehyde, air-dried, and incubated for 20 min in PBS supplemented with 10% normal goat serum. Sections were incubated in a 1:50 dilution of Rockland's rabbit anti-Mouse IL-18 antibody or 1 µg/ml nonimmune rabbit IgG (not shown) as negative control. The antibodies were diluted in PBS containing 1% bovine serum albumin. After an overnight incubation at 4°C, the sections were washed three times with 0.5% bovine serum albumin in PBS. The sections were then incubated with a secondary goat anti-rabbit antibody conjugated to Alexa488 (Molecular Probes) for 60 min at room temperature in the dark. Nuclei were counterstained blue using 1 µg/100 ml bisbenzimide. After staining, sections were washed and examined with the Leica DM RXA confocal laser scanning system and analyzed. Similar staining will occur with other systems.

Sample type

IF Microscopy