Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C745265 - Provider product page
- Provider
- LSBio
- Product name
- IL18 Antibody LS-C745265
- Antibody type
- Polyclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Immunohistochemistry with Rabbit anti-Mouse IL-18 antibody showing IL-18 staining in inflammatory cells of the mucous corium of mouse colon at 20x and 40x. Slide A is a negative control. Slides B and C show staining. Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with mouse anti-IL-18 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 4 universal AP polymer detection system and visualized with WARP RED.