RP1017

antibody from Boster Biological Technology

Targeting: IL18 IGIF, IL-18, IL-1g, IL1F4

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

RP1017

Provider

Boster Biological Technology

Product name

Anti-Interleukin-18 IL18 Antibody

Antibody type

Polyclonal

Description

Polyclonal antibody for IL 1F4/IL18 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Mouse. IL 1F4/IL18 information: Molecular Weight: 22135 MW; Subcellular Localization: Secreted.

Reactivity

Mouse

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of IL-18 using anti- IL-18 antibody (RP1017). IL-18 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- IL-18 Antibody (RP1017) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image