PA5-72848

antibody from Invitrogen Antibodies

Targeting: MYH3 HEMHC, MYHC-EMB, MYHSE1, SMHCE

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-72848

Provider

Invitrogen Antibodies

Product name

MYH3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MYH3 in fetal human skeletal muscle lysate with MYH3 Polyclonal Antibody (Product # PA5-72848) at 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-MYH3 Polyclonal Antibody (Product # PA5-72848) and a 230 kDa band corresponding to MYH3 was observed in Rat heart in comparison to Rat testis which is reported to be negative. Tissue extracts (30 µg lysate) of Rat Heart (Lane 1) and Rat Testis (Lane 2) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of MYH3 in mouse skeletal muscle tissue with MYH3 Polyclonal Antibody (Product # PA5-72848) at 5 µg/mL.