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- References [13]
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- Immunohistochemistry [1]
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- Product number
- AF885 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Gas6 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Gas6 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse Gas6 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_000811- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology.
Gas6 derived from cancer-associated fibroblasts promotes migration of Axl-expressing lung cancer cells during chemotherapy.
AXL-GAS6 expression can predict for adverse prognosis in non-small cell lung cancer with brain metastases.
Sympathetic Signaling Reactivates Quiescent Disseminated Prostate Cancer Cells in the Bone Marrow.
Expansion of necrotic core and shedding of Mertk receptor in human carotid plaques: a role for oxidized polyunsaturated fatty acids?
Identification of Axl as a downstream effector of TGF-β1 during Langerhans cell differentiation and epidermal homeostasis.
Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques.
Expression of the vitamin K-dependent proteins GAS6 and protein S and the TAM receptor tyrosine kinases in human atherosclerotic carotid plaques.
GAS6/Mer axis regulates the homing and survival of the E2A/PBX1-positive B-cell precursor acute lymphoblastic leukemia in the bone marrow niche.
Plasma protein growth arrest-specific 6 levels are associated with altered glucose tolerance, inflammation, and endothelial dysfunction.
Up-regulation of soluble Axl and Mer receptor tyrosine kinases negatively correlates with Gas6 in established multiple sclerosis lesions.
Gas6 evaluation in patients with acute dyspnea due to suspected pulmonary embolism.
Fujita K, Chen X, Homma H, Tagawa K, Amano M, Saito A, Imoto S, Akatsu H, Hashizume Y, Kaibuchi K, Miyano S, Okazawa H
Nature communications 2018 Jan 30;9(1):433
Nature communications 2018 Jan 30;9(1):433
Gas6 derived from cancer-associated fibroblasts promotes migration of Axl-expressing lung cancer cells during chemotherapy.
Kanzaki R, Naito H, Kise K, Takara K, Eino D, Minami M, Shintani Y, Funaki S, Kawamura T, Kimura T, Okumura M, Takakura N
Scientific reports 2017 Sep 6;7(1):10613
Scientific reports 2017 Sep 6;7(1):10613
AXL-GAS6 expression can predict for adverse prognosis in non-small cell lung cancer with brain metastases.
Wu X, Ma W, Zhou Q, Yan H, Lim ZF, Huang M, Deng C, Yu X, Su H, Komo S, Yang H, Zhang X, Wen S, Zhang Z, Ma PC
Journal of cancer research and clinical oncology 2017 Oct;143(10):1947-1957
Journal of cancer research and clinical oncology 2017 Oct;143(10):1947-1957
Sympathetic Signaling Reactivates Quiescent Disseminated Prostate Cancer Cells in the Bone Marrow.
Decker AM, Jung Y, Cackowski FC, Yumoto K, Wang J, Taichman RS
Molecular cancer research : MCR 2017 Dec;15(12):1644-1655
Molecular cancer research : MCR 2017 Dec;15(12):1644-1655
Expansion of necrotic core and shedding of Mertk receptor in human carotid plaques: a role for oxidized polyunsaturated fatty acids?
Garbin U, Baggio E, Stranieri C, Pasini A, Manfro S, Mozzini C, Vallerio P, Lipari G, Merigo F, Guidi G, Cominacini L, Fratta Pasini A
Cardiovascular research 2013 Jan 1;97(1):125-33
Cardiovascular research 2013 Jan 1;97(1):125-33
Identification of Axl as a downstream effector of TGF-β1 during Langerhans cell differentiation and epidermal homeostasis.
Bauer T, Zagórska A, Jurkin J, Yasmin N, Köffel R, Richter S, Gesslbauer B, Lemke G, Strobl H
The Journal of experimental medicine 2012 Oct 22;209(11):2033-47
The Journal of experimental medicine 2012 Oct 22;209(11):2033-47
Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
Zizzo G, Hilliard BA, Monestier M, Cohen PL
Journal of immunology (Baltimore, Md. : 1950) 2012 Oct 1;189(7):3508-20
Journal of immunology (Baltimore, Md. : 1950) 2012 Oct 1;189(7):3508-20
Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques.
Clauser S, Meilhac O, Bièche I, Raynal P, Bruneval P, Michel JB, Borgel D
Thrombosis and haemostasis 2012 Jan;107(1):140-9
Thrombosis and haemostasis 2012 Jan;107(1):140-9
Expression of the vitamin K-dependent proteins GAS6 and protein S and the TAM receptor tyrosine kinases in human atherosclerotic carotid plaques.
Hurtado B, Muñoz X, Recarte-Pelz P, García N, Luque A, Krupinski J, Sala N, García de Frutos P
Thrombosis and haemostasis 2011 May;105(5):873-82
Thrombosis and haemostasis 2011 May;105(5):873-82
GAS6/Mer axis regulates the homing and survival of the E2A/PBX1-positive B-cell precursor acute lymphoblastic leukemia in the bone marrow niche.
Shiozawa Y, Pedersen EA, Taichman RS
Experimental hematology 2010 Feb;38(2):132-40
Experimental hematology 2010 Feb;38(2):132-40
Plasma protein growth arrest-specific 6 levels are associated with altered glucose tolerance, inflammation, and endothelial dysfunction.
Hung YJ, Lee CH, Chu NF, Shieh YS
Diabetes care 2010 Aug;33(8):1840-4
Diabetes care 2010 Aug;33(8):1840-4
Up-regulation of soluble Axl and Mer receptor tyrosine kinases negatively correlates with Gas6 in established multiple sclerosis lesions.
Weinger JG, Omari KM, Marsden K, Raine CS, Shafit-Zagardo B
The American journal of pathology 2009 Jul;175(1):283-93
The American journal of pathology 2009 Jul;175(1):283-93
Gas6 evaluation in patients with acute dyspnea due to suspected pulmonary embolism.
Sainaghi PP, Alciato F, Carnieletto S, Castello L, Bergamasco L, Sola D, Bongo AS, Inglese E, Polosa R, Avanzi GC
Respiratory medicine 2009 Apr;103(4):589-94
Respiratory medicine 2009 Apr;103(4):589-94
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Gas6 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, SW480 human colorectal adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and human intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF885) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Gas6 at approximately 64 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Gas6 in Human Stomach Cancer Tissue. Gas6 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using 10 µg/mL Goat Anti-Human Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF885) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
