DM3525

antibody from Acris Antibodies GmbH

Targeting: PGF D12S1900, PGFL, PIGF, PLGF, PlGF-2, SHGC-10760

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

DM3525

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#DM3525, RRID:AB_1770607

Product name

anti Placenta growth factor / PGF (PlGF-1/2)

Antibody type

Monoclonal

Antigen

Recombinant Human PlGF-2 [Leu19 – Arg170] (produced in insect cells)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

178/G10

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis: Detection of PlGF in Human placenta lysates.a) Patient 1b) Patient 2 (50 µg lysate/lane).DM3525 (178/G10) was used at 2 µg/ml as first antibody. AP-conjugated Goat anti Mouse was used as a secondary antibody.

ELISA

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

PlGF Sandwich-ELISA using recombinant human PlGF-1 as standard (Cat.-No DA3508). Mouse anti-Human PlGF #178/G10 (Cat.-No DM3525) was used as capture antibody, Biotinylated rabbit anti-human PlGF was used for detection.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence with anti-Human PlGF antibodies (clones G10 and 3B10): Sf9 insect cells were infected with recombinant Baculovirus for Human PlGF-1 and PlGF-2. Three days after infection the Immunofluorescence protocol was performed.

Immunoprecipitation

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunprecipitation of rh-PlGF-1 using monoclonal anti-Human PlGF antibody (clone 178/G10 Cat#DM3525)