AF-264-PB

antibody from R&D Systems

Targeting: PGF D12S1900, PGFL, PIGF, PLGF, PlGF-2, SHGC-10760

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF-264-PB

Provider

R&D Systems

Product name

Human PlGF Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human PlGF (but not VEGF) in direct ELISAs and Western blots. This antibody will also recognize the heterodimer of VEGF and PlGF. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse PlGF-2 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

CAA38698


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.

Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST
Angiogenesis 2010 Dec;13(4):337-47

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VEGF-A and VEGF-F evoke distinct changes in vascular ultrastructure.

Matsunaga Y, Yamazaki Y, Suzuki H, Morita T
Biochemical and biophysical research communications 2009 Feb 20;379(4):872-5

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Malignant mesothelioma growth inhibition by agents that target the VEGF and VEGF-C autocrine loops.

Masood R, Kundra A, Zhu S, Xia G, Scalia P, Smith DL, Gill PS
International journal of cancer 2003 May 1;104(5):603-10

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Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome.

Zhou Y, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ
The American journal of pathology 2002 Apr;160(4):1405-23

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human PlGF by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with full length human PlGF. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PlGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-264-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PlGF at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.