MAB6298

antibody from R&D Systems

Targeting: MUC1 ADMCKD, ADMCKD1, CD227, MCD, MCKD, MCKD1, PEM, PUM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6298

Provider

R&D Systems

Product name

Human MUC-1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human MUC-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) MUC-20, rhMUC-20S, or rhCA125/MUC-16 was observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P15941


Isotype

IgG

Antibody clone number

604804

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

IL4 Primes the Dynamics of Breast Cancer Progression via DUSP4 Inhibition.

Gaggianesi M, Turdo A, Chinnici A, Lipari E, Apuzzo T, Benfante A, Sperduti I, Di Franco S, Meraviglia S, Lo Presti E, Dieli F, Caputo V, Militello G, Vieni S, Stassi G, Todaro M
Cancer research 2017 Jun 15;77(12):3268-3279

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human MUC-1 by Western Blot. Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MUC-1 at approximately 300 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human MUC-1 by Simple Western^TM. Simple Western lane view shows lysates of human lung tissue, loaded at 0.2 mg/mL. Specific bands were detected for MUC-1 at approximately 300-500 kDa (as indicated) using 40 µg/mL of Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

MUC-1 in Capan-1 Human Cell Line. MUC-1 was detected in immersion fixed Capan-1 human pancreatic adenocarcinoma cell line using Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

MUC-1 in Human Breast. MUC-1 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of MUC-1 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂ Secondary Antibody (Catalog # F0101B).