Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00005122-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005122-M02, RRID:AB_829948
- Product name
- Mouse Monoclonal Proprotein Convertase 1/PCSK1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PCSK1 (3D2)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Urocortin 3 marks mature human primary and embryonic stem cell-derived pancreatic alpha and beta cells.
van der Meulen T, Xie R, Kelly OG, Vale WW, Sander M, Huising MO
PloS one 2012;7(12):e52181
PloS one 2012;7(12):e52181
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - PCSK1 monoclonal antibody (M02), clone 3D2. Analysis of PCSK1 expression in PC-12.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - PCSK1 monoclonal antibody (M02), clone 3D2 Analysis of PCSK1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Detection limit for recombinant GST tagged PCSK1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on HeLa cell. Antibody concentration 30 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.