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Learn710368
antibody from Invitrogen Antibodies
Targeting: TNFRSF1A
CD120a, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR60
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 710368 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNFR1 Recombinant Polyclonal Antibody (12HCLC)
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody is predicted to react with mouse, rat and rabbit based on sequence homology.
- Antibody clone number
- 12HCLC
- Concentration
- 0.5 mg/mL
Submitted references Immune heterogeneity and clinicopathologic characterization of IGFBP2 in 2447 glioma samples.
Cai J, Chen Q, Cui Y, Dong J, Chen M, Wu P, Jiang C
Oncoimmunology 2018;7(5):e1426516
Oncoimmunology 2018;7(5):e1426516
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TNF Receptor I in whole cell extracts from COS7 cells using a TNF Receptor I Recombinant Rabbit Polyclonal Antibody (Product # 710368) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~22kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of STNFR1 showing staining in the cytoplasm and weak staining in the nucleus of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a STNFR1 Recombinant Rabbit Polyclonal Antibody (Product # 710368) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
