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antibody from Invitrogen Antibodies
Targeting: NCOA6
AIB3, ASC2, KIAA0181, NRC, PRIP, RAP250, TRBP
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- PA5-52071 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCOA6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NQNVQHAGGQ GAGPPQNQMQ VSHGPPNMMQ PSLMGIHGNM NNQQAGTSGV PQVNLSNMQG QPQQGPPSQL MGMHQQIVPS QGQMVQQQGT LNPQNPMILS RAQLMPQGQM MVNPPSQNLG PSPQRMTPPK Q
- Concentration
- 0.1 mg/mL
Submitted references Nuclear receptor coactivator 6 promotes HTR-8/SVneo cell invasion and migration by activating NF-κB-mediated MMP9 transcription.
Wu L, Zhao KQ, Wang W, Cui LN, Hu LL, Jiang XX, Shuai J, Sun YP
Cell proliferation 2020 Sep;53(9):e12876
Cell proliferation 2020 Sep;53(9):e12876
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NCOA6 in human cell line U-251 MG shows positivity in vesicles & nucleus but excluded from the nucleoli. Samples were probed using a NCOA6 Polyclonal Antibody (Product # PA5-52071).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCOA6 in human testis and skeletal muscle tissues using NCOA6 Polyclonal Antibody (Product # PA5-52071). Corresponding NCOA6 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCOA6 in human testis using NCOA6 Polyclonal Antibody (Product # PA5-52071) shows moderate to strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCOA6 in human placenta using NCOA6 Polyclonal Antibody (Product # PA5-52071) shows moderate to strong nuclear positivity in trophoblastic cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCOA6 in human fallopian tube using NCOA6 Polyclonal Antibody (Product # PA5-52071) shows moderate to strong nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCOA6 in human skeletal muscle using NCOA6 Polyclonal Antibody (Product # PA5-52071) shows weak nuclear positivity in myocytes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 NCOA6 knockdown significantly impaired the invasion and migration of HTR-8/SVneo cells. A, Significantly attenuated invasion and migration occurred after the transfection of NCOA6 siRNAs into HTR-8/SVneo cells. All invaded/migrated cells outside the insert membrane were stained and photographed (left panel; scale bars: 1 mm). The fold change in cell invasion/migration capacity after treatment was estimated by counting the invaded/migrated cells with staining (right panel). B, In parallel with the transwell assays, CCK-8 assays were carried out to determine the change in the number of cells 0, 24, 48 and 72 hours after transfection. C, D, The mRNA and protein levels of NCOA6 were examined after siRNA transfection. A representative image from a Western blot is shown, and the molecular weight markers are indicated on the left in kDa. All results are presented as the mean +- SD based on at least three independent experiments. ** P < .01
