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LearnNB200-335
antibody from Novus Biologicals
Targeting: NCOA6
AIB3, ASC2, KIAA0181, NRC, PRIP, RAP250, TRBP
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NB200-335 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-335, RRID:AB_10001407
- Product name
- Rabbit Polyclonal NCOA6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references p19Arf inhibits aggressive progression of H-ras-driven hepatocellular carcinoma.
Neonatal activation of the nuclear receptor CAR results in epigenetic memory and permanent change of drug metabolism in mouse liver.
Four enzymes cooperate to displace histone H1 during the first minute of hormonal gene activation.
Crucial roles for interactions between MLL3/4 and INI1 in nuclear receptor transactivation.
Identification and characterization of a novel nuclear protein complex involved in nuclear hormone receptor-mediated gene regulation.
Targeted inactivation of MLL3 histone H3-Lys-4 methyltransferase activity in the mouse reveals vital roles for MLL3 in adipogenesis.
PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex.
Kopanja D, Huang S, Al Raheed MRH, Guzman G, Raychaudhuri P
Carcinogenesis 2018 Mar 8;39(3):318-326
Carcinogenesis 2018 Mar 8;39(3):318-326
Neonatal activation of the nuclear receptor CAR results in epigenetic memory and permanent change of drug metabolism in mouse liver.
Chen WD, Fu X, Dong B, Wang YD, Shiah S, Moore DD, Huang W
Hepatology (Baltimore, Md.) 2012 Oct;56(4):1499-509
Hepatology (Baltimore, Md.) 2012 Oct;56(4):1499-509
Four enzymes cooperate to displace histone H1 during the first minute of hormonal gene activation.
Vicent GP, Nacht AS, Font-Mateu J, Castellano G, Gaveglia L, Ballaré C, Beato M
Genes & development 2011 Apr 15;25(8):845-62
Genes & development 2011 Apr 15;25(8):845-62
Crucial roles for interactions between MLL3/4 and INI1 in nuclear receptor transactivation.
Lee S, Kim DH, Goo YH, Lee YC, Lee SK, Lee JW
Molecular endocrinology (Baltimore, Md.) 2009 May;23(5):610-9
Molecular endocrinology (Baltimore, Md.) 2009 May;23(5):610-9
Identification and characterization of a novel nuclear protein complex involved in nuclear hormone receptor-mediated gene regulation.
Garapaty S, Xu CF, Trojer P, Mahajan MA, Neubert TA, Samuels HH
The Journal of biological chemistry 2009 Mar 20;284(12):7542-52
The Journal of biological chemistry 2009 Mar 20;284(12):7542-52
Targeted inactivation of MLL3 histone H3-Lys-4 methyltransferase activity in the mouse reveals vital roles for MLL3 in adipogenesis.
Lee J, Saha PK, Yang QH, Lee S, Park JY, Suh Y, Lee SK, Chan L, Roeder RG, Lee JW
Proceedings of the National Academy of Sciences of the United States of America 2008 Dec 9;105(49):19229-34
Proceedings of the National Academy of Sciences of the United States of America 2008 Dec 9;105(49):19229-34
PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex.
Cho YW, Hong T, Hong S, Guo H, Yu H, Kim D, Guszczynski T, Dressler GR, Copeland TD, Kalkum M, Ge K
The Journal of biological chemistry 2007 Jul 13;282(28):20395-406
The Journal of biological chemistry 2007 Jul 13;282(28):20395-406
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCOA6 Antibody [NB200-335] - Detection of Human ASC2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-335 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NCOA6 Antibody [NB200-335] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASC2 antibody used for IP at 6 ug per reaction. ASC2 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-ASC2 antibody. For blotting immunoprecipitated ASC2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NCOA6 Antibody [NB200-335] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASC2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
