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- Antibody Data
- Antigen structure
- References [21]
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- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- BAF452 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CXCL2/GRO beta/MIP-2/CINC-3 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CXCL2/MIP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) GRO beta , rhGRO gamma , recombinant mouse (rm) C10, rmMIP-1 alpha , rmMIP-1 beta , rhRANTES, and rmKC is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P10889- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Modulation of double-stranded RNA pattern recognition receptor signaling in ovarian cancer cells promotes inflammatory queues.
IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3.
The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection.
Pulmonary Epithelial Cell-Derived Cytokine TGF-β1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling.
ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Sequential ELISA to profile multiple cytokines from small volumes.
Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
Muccioli M, Nandigam H, Loftus T, Singh M, Venkatesh A, Wright J, Pate M, McCall K, Benencia F
Oncotarget 2018 Nov 30;9(94):36666-36683
Oncotarget 2018 Nov 30;9(94):36666-36683
IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3.
Lv R, Zhao J, Lei M, Xiao D, Yu Y, Xie J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(5):1961-1972
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(5):1961-1972
The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection.
Pang Z, Junkins RD, MacNeil AJ, McCormick C, Cheng Z, Chen WM, Lin TJ
Journal of leukocyte biology 2017 Dec;102(6):1461-1469
Journal of leukocyte biology 2017 Dec;102(6):1461-1469
Pulmonary Epithelial Cell-Derived Cytokine TGF-β1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
Denney L, Byrne AJ, Shea TJ, Buckley JS, Pease JE, Herledan GM, Walker SA, Gregory LG, Lloyd CM
Immunity 2015 Nov 17;43(5):945-58
Immunity 2015 Nov 17;43(5):945-58
Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
Junkins RD, MacNeil AJ, Wu Z, McCormick C, Lin TJ
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5178-86
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5178-86
Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
The American journal of pathology 2011 Dec;179(6):2730-9
The American journal of pathology 2011 Dec;179(6):2730-9
Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling.
Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
The Journal of biological chemistry 2009 Sep 18;284(38):25900-11
The Journal of biological chemistry 2009 Sep 18;284(38):25900-11
ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
Microbes and infection 2009 Apr;11(4):524-30
Microbes and infection 2009 Apr;11(4):524-30
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
Immunology 2008 Oct;125(2):272-9
Immunology 2008 Oct;125(2):272-9
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):612-20
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):612-20
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):612-20
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):612-20
Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjöstedt A, Edebro H, Forsman M, Byström M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
Infection and immunity 2006 Dec;74(12):6730-8
Infection and immunity 2006 Dec;74(12):6730-8
Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 15;177(12):8650-7
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 15;177(12):8650-7
Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 15;177(12):8650-7
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 15;177(12):8650-7
Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
European journal of immunology 2006 Aug;36(8):2025-34
European journal of immunology 2006 Aug;36(8):2025-34
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Osuchowski MF, Welch K, Siddiqui J, Remick DG
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Osuchowski MF, Welch K, Siddiqui J, Remick DG
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Sequential ELISA to profile multiple cytokines from small volumes.
Osuchowski MF, Siddiqui J, Copeland S, Remick DG
Journal of immunological methods 2005 Jul;302(1-2):172-81
Journal of immunological methods 2005 Jul;302(1-2):172-81
Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noël A, Cataldo DD
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 15;175(4):2589-97
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 15;175(4):2589-97
Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noël A, Cataldo DD
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 15;175(4):2589-97
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 15;175(4):2589-97
Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
Jeyaseelan S, Chu HW, Young SK, Worthen GS
Infection and immunity 2004 Dec;72(12):7247-56
Infection and immunity 2004 Dec;72(12):7247-56
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCL2/GRO beta/MIP-2/CINC-3 in Mouse Splenocytes. CXCL2/GRO beta/MIP-2/CINC-3 was detected in immersion fixed mouse splenocytes stimulated with LPS and monensin using Mouse CXCL2/GRO beta/MIP-2/CINC-3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF452) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCL2/GRO beta/MIP-2/CINC-3 in Mouse Spleen. CXCL2/GRO beta/MIP-2/CINC-3 was detected in immersion fixed frozen sections of mouse spleen using Mouse CXCL2/GRO beta/MIP-2/CINC-3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF452) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
