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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- AF2644 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Gas1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Gas1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human Gas1 and recombinant mouse Gas6 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Growth Arrest Specific 1 (Gas1) Gene Overexpression in Liver Reduces the In Vivo Progression of Murine Hepatocellular Carcinoma and Partially Restores Gene Expression Levels.
Gas1 is a receptor for sonic hedgehog to repel enteric axons.
Growth arrest-specific protein 1 is a novel endogenous inhibitor of glomerular cell activation and proliferation.
Epigenetic transcriptional regulation of the growth arrest-specific gene 1 (Gas1) in hepatic cell proliferation at mononucleosomal resolution.
Sacilotto N, Castillo J, Riffo-Campos ÁL, Flores JM, Hibbitt O, Wade-Martins R, López C, Rodrigo MI, Franco L, López-Rodas G
PloS one 2015;10(7):e0132477
PloS one 2015;10(7):e0132477
Gas1 is a receptor for sonic hedgehog to repel enteric axons.
Jin S, Martinelli DC, Zheng X, Tessier-Lavigne M, Fan CM
Proceedings of the National Academy of Sciences of the United States of America 2015 Jan 6;112(1):E73-80
Proceedings of the National Academy of Sciences of the United States of America 2015 Jan 6;112(1):E73-80
Growth arrest-specific protein 1 is a novel endogenous inhibitor of glomerular cell activation and proliferation.
van Roeyen CR, Zok S, Pruessmeyer J, Boor P, Nagayama Y, Fleckenstein S, Cohen CD, Eitner F, Gröne HJ, Ostendorf T, Ludwig A, Floege J
Kidney international 2013 Feb;83(2):251-63
Kidney international 2013 Feb;83(2):251-63
Epigenetic transcriptional regulation of the growth arrest-specific gene 1 (Gas1) in hepatic cell proliferation at mononucleosomal resolution.
Sacilotto N, Espert A, Castillo J, Franco L, López-Rodas G
PloS one 2011;6(8):e23318
PloS one 2011;6(8):e23318
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Gas1 in Mouse Embryo. Gas1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Gas1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2644) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
