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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-48298 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GAS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 400 µL
- Concentration
- 1.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer.
Lin LL, Kost ER, Lin CL, Valente P, Wang CM, Kolonin MG, Daquinag AC, Tan X, Lucio N, Hung CN, Wang CP, Kirma NB, Huang TH
Cell reports 2020 Oct 13;33(2):108253
Cell reports 2020 Oct 13;33(2):108253
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GAS1 in NCI-H460 cell line lysates (35 µg/lane). Lysates were probed with a GAS1 Antibody (N-term) (Product # PA5-48298).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of GAS1 in NCI-H460 cells. Cells were stained with a GAS1 Antibody (N-term) (Product # PA5-48298) followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GAS1 in formalin fixed and paraffin embedded mouse heart tissue tissue. Samples were probed with a GAS1 Antibody (N-term) (Product # PA5-48298) followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of GAS1 in NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). Samples were probed with a GAS1 Antibody (N-term) (Product # PA5-48298). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
