M03515

antibody from Boster Biological Technology

Targeting: NONO NMT55, NRB54, P54, P54NRB, PPP1R114

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

M03515

Provider

Boster Biological Technology

Product name

Anti-nmt55 p54nrb NONO Antibody Picoband™ (monoclonal, 11E2)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for nmt55 p54nrb detection. Tested with WB, ICC, ICC/IF, FCM in Human.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1.10E+03

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of U20S cells using anti-nmt55 p54nrb antibody (M03515). Overlay histogram showing U20S cells stained with M03515 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-nmt55 p54nrb Antibody (M03515,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image