RQ4624

antibody from NSJ Bioreagents

Targeting: NONO NMT55, NRB54, P54, P54NRB, PPP1R114

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ4624

Provider

NSJ Bioreagents

Product name

NONO Antibody / p54nrb

Antibody type

Monoclonal

Description

Protein G affinity

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Vial size

100 µg

Storage

After reconstitution, the NONO antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) placenta, 3) MCF7, 4) A549, 5) SW620, 6) PANC-1, 7) U-2 OS and 8) K562 lysate with NONO antibody at 0.5ug/ml. Predicted molecular weight ~54 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ICC staining of FFPE human A431 cells with NONO antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U-2 OS cells with NONO antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NONO antibody.