11058-1-AP
antibody from Invitrogen Antibodies
Targeting: NONO
NMT55, NRB54, P54, P54NRB, PPP1R114
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 11058-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NONO Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: IAKVELDNM PLRGKQLRVR FACHSASLTV RNLPQYVSNE LLEEAFSVFG QVERAVVIVD DRGRPSGKGI VEFSGKPAAR KALDRCSEGS FLLTTFPRPV TVEPMDQLDD EEGLPEKLVI KNQQFHKERE QPPRFAQPGS FEYEYAMRWK ALIEMEKQQQ DQVDRNIKEA REKLEMEMEA ARHEHQVMLM RQDLMRRQEE LRRMEELHNQ EVQKRKQLEL RQEEERRRRE EEMRRQQEEM MRRQQEGFKG TFPDAREQEI RMGQMAMGGA MGINNRGAMP PAPVPAGTPA PPGPATMMPD GTLGLTPPTT ERFGQAATME GIGAIGGTPP AFNRAAPGAE FAPNKRRRY (124-471 aa encoded by BC010049)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.32 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using NONO antibody 11058-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 11058-1-AP (NONO antibody) at dilution of 1:50 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with 0.2ug NONO antibody (11058-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-NONO (IP:11058-1-AP, 4ug; Detection:11058-1-AP 1:1000) with HEK-293 cells lysate 2800ug.