Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
Reference
Comment
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- Product number
- TA307281 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit monoclonal antibody against ROR2(clone EPR3779)
- Antibody type
- Monoclonal
- Description
- Rabbit monoclonal antibody against ROR2(clone EPR3779)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ROR2
- Isotype
- IgG
- Antibody clone number
- EPR3779
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references Wnt5A regulates the expression of ROR2 tyrosine kinase receptor in ovarian cancer cells.
Hossein G, Arabzadeh S, Salehi-Dulabi Z, Dehghani-Ghobadi Z, Heidarian Y, Talebi-Juybari M
Biochemistry and cell biology = Biochimie et biologie cellulaire 2017 Dec;95(6):609-615
Biochemistry and cell biology = Biochimie et biologie cellulaire 2017 Dec;95(6):609-615
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot - ROR2 antibody [EPR3779]; All lanes : Anti-ROR2 antibody [EPR3779] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : MCF-7 cell lysate.Lane 3 : SH-SY5Y cell lysate.Lane 4 : Saos-2 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit. at 1/2000 dilution.Predicted band size : 105 kDa.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IHC (Frozen sections) - Anti-ROR2 antibody; TA307281 staining ROR2 in Mouse cartilage precursor to endochondral bone tissue by IHC (Frozen sections). The sections were PFA-fixed prior to blocking with 20% serum for 1 hour at 19°C and permeabilization with 0.1% Triton. The primary antibody was diluted 1/50 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor? 647-conjugated Rabbit polyclonal was used as the secondary antibody, diluted 1/200. DAPI was used for the blue counterstain.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry - ROR2 antibody [EPR3779]; Flow cytometric analysis of Hela cells using TA307281 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
- Validation comment
- FC