Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF2064 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ROR2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human ROR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human ROR1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAA60276
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Receptor tyrosine kinase-like orphan receptor 2 (Ror2) expression creates a poised state of Wnt signaling in renal cancer.
Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex.
Therapeutic potential and challenges of targeting receptor tyrosine kinase ROR1 with monoclonal antibodies in B-cell malignancies.
Wnt5a/Ror2-induced upregulation of xPAPC requires xShcA.
The Wnt-5a-derived hexapeptide Foxy-5 inhibits breast cancer metastasis in vivo by targeting cell motility.
Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia.
Rasmussen NR, Wright TM, Brooks SA, Hacker KE, Debebe Z, Sendor AB, Walker MP, Major MB, Green J, Wahl GM, Rathmell WK
The Journal of biological chemistry 2013 Sep 6;288(36):26301-26310
The Journal of biological chemistry 2013 Sep 6;288(36):26301-26310
Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex.
Endo M, Doi R, Nishita M, Minami Y
Journal of cell science 2012 Apr 15;125(Pt 8):2017-29
Journal of cell science 2012 Apr 15;125(Pt 8):2017-29
Therapeutic potential and challenges of targeting receptor tyrosine kinase ROR1 with monoclonal antibodies in B-cell malignancies.
Yang J, Baskar S, Kwong KY, Kennedy MG, Wiestner A, Rader C
PloS one 2011;6(6):e21018
PloS one 2011;6(6):e21018
Wnt5a/Ror2-induced upregulation of xPAPC requires xShcA.
Feike AC, Rachor K, Gentzel M, Schambony A
Biochemical and biophysical research communications 2010 Oct 1;400(4):500-6
Biochemical and biophysical research communications 2010 Oct 1;400(4):500-6
The Wnt-5a-derived hexapeptide Foxy-5 inhibits breast cancer metastasis in vivo by targeting cell motility.
Säfholm A, Tuomela J, Rosenkvist J, Dejmek J, Härkönen P, Andersson T
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Oct 15;14(20):6556-63
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Oct 15;14(20):6556-63
Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia.
Baskar S, Kwong KY, Hofer T, Levy JM, Kennedy MG, Lee E, Staudt LM, Wilson WH, Wiestner A, Rader C
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jan 15;14(2):396-404
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jan 15;14(2):396-404
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human ROR2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human ROR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2064) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ROR2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human ROR2 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ROR2 at approximately 139 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ROR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2064) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.