Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF538 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat MAG/Siglec-4a Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat MAG/Siglec-4a in direct ELISAs and Western blots.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P07722- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Nonneuronal control of the differential distribution of myelin along retinal ganglion cell axons in the mouse.
In vitro expanded stem cells from the developing retina fail to generate photoreceptors but differentiate into myelinating oligodendrocytes.
Cleavage of myelin associated glycoprotein by matrix metalloproteinases.
Netrin-1 is a novel myelin-associated inhibitor to axon growth.
Neuregulin 1-erbB signaling is necessary for normal myelination and sensory function.
Yang X, Zou H, Jung G, Richard G, Linke SJ, Ader M, Bartsch U
Investigative ophthalmology & visual science 2013 Dec 2;54(13):7819-27
Investigative ophthalmology & visual science 2013 Dec 2;54(13):7819-27
In vitro expanded stem cells from the developing retina fail to generate photoreceptors but differentiate into myelinating oligodendrocytes.
Czekaj M, Haas J, Gebhardt M, Müller-Reichert T, Humphries P, Farrar J, Bartsch U, Ader M
PloS one 2012;7(7):e41798
PloS one 2012;7(7):e41798
Cleavage of myelin associated glycoprotein by matrix metalloproteinases.
Milward E, Kim KJ, Szklarczyk A, Nguyen T, Melli G, Nayak M, Deshpande D, Fitzsimmons C, Hoke A, Kerr D, Griffin JW, Calabresi PA, Conant K
Journal of neuroimmunology 2008 Jan;193(1-2):140-8
Journal of neuroimmunology 2008 Jan;193(1-2):140-8
Netrin-1 is a novel myelin-associated inhibitor to axon growth.
Löw K, Culbertson M, Bradke F, Tessier-Lavigne M, Tuszynski MH
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Jan 30;28(5):1099-108
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Jan 30;28(5):1099-108
Neuregulin 1-erbB signaling is necessary for normal myelination and sensory function.
Chen S, Velardez MO, Warot X, Yu ZX, Miller SJ, Cros D, Corfas G
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Mar 22;26(12):3079-86
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Mar 22;26(12):3079-86
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MAG/Siglec-4a in Rat Brain. MAG/Siglec-4a was detected in perfusion fixed frozen sections of rat brain (cerebellum) using 25 µg/mL Goat Anti-Rat MAG/Siglec-4a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF538) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to processes of oligodendrocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
