PA5-22128

antibody from Invitrogen Antibodies

Targeting: AIP ARA9, FKBP16, FKBP37, XAP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22128

Provider

Invitrogen Antibodies

Product name

AIP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, Molt-4, Raji, mouse brain, PC-12, Rat2.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AIP Polyclonal Antibody detects ARA9 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract. B. 30 µg Molt-4 whole cell lysate/extract. C. 30 µg Raji whole cell lysate/extract.10 % SDS-PAGE. AIP Polyclonal Antibody (Product # PA5-22128) dilution: 1:5,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of AIP was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a AIP Polyclonal Antibody (Product # PA5-22128) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using AIP Polyclonal Antibody (Product # PA5-22128). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. AIP Polyclonal Antibody (Product # PA5-22128) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AIP Polyclonal Antibody detects ARA9 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract. B. 30 µg Molt-4 whole cell lysate/extract. C. 30 µg Raji whole cell lysate/extract.10 % SDS-PAGE. AIP Polyclonal Antibody (Product # PA5-22128) dilution: 1:5,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of XAP2 in paraformaldehyde-fixed HeLa cells using a XAP2 polyclonal antibody (Product # PA5-22128) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ARA9 antibody (Product # PA5-22128) at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AIP Polyclonal Antibody detects ARA9 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. AIP Polyclonal Antibody (Product # PA5-22128) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.