GTX79473

antibody from GeneTex

Targeting: AKAP9 AKAP350, AKAP450, CG-NAP, HYPERION, KIAA0803, LQT11, MU-RMS-40.16A, PPP1R45, PRKA9, YOTIAO

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX79473

Provider

GeneTex

Proper citation

GeneTex Cat#GTX79473, RRID:AB_11174025

Product name

AKAP9 antibody [17G10]

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of AKAP450 in A431 cells. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with AKAP450 antibody [17G10] at a dilution of 1:100 overnight at 4°C, washed with PBS and incubated with a proper secondary antibody. AKAP450 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemistry was performed on normal biopsies of deparaffinized human pancreas tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed at a dilution of 1:20 with or without AKAP450 antibody [17G10] overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjμgated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.