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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- MA5-23522 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BICD2 Monoclonal Antibody (3I3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, A431, HeLa, and HepG2 cells.
- Antibody clone number
- 3I3
- Concentration
- 1 mg/mL
Submitted references Cytoplasmic control of intranuclear polarity by human cytomegalovirus.
MiR-4674 regulates angiogenesis in tissue injury by targeting p38K signaling in endothelial cells.
Procter DJ, Furey C, Garza-Gongora AG, Kosak ST, Walsh D
Nature 2020 Nov;587(7832):109-114
Nature 2020 Nov;587(7832):109-114
MiR-4674 regulates angiogenesis in tissue injury by targeting p38K signaling in endothelial cells.
Icli B, Li H, Pérez-Cremades D, Wu W, Ozdemir D, Haemmig S, Guimaraes RB, Manica A, Marchini JF, Orgill DP, Feinberg MW
American journal of physiology. Cell physiology 2020 Mar 1;318(3):C524-C535
American journal of physiology. Cell physiology 2020 Mar 1;318(3):C524-C535
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BICD2 in various whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with a BICD2 monoclonal antibody (Product # MA5-23522) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BICD2 Polyclonal Antibody detects BICD2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 Polyclonal Antibody BICD2 Monoclonal Antibody (3I3) (Product # MA5-23522) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of BICD2 was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a BICD2 Monoclonal Antibody (3I3) (Product # MA5-23522) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BICD2 Polyclonal Antibody detects BICD2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 Polyclonal Antibody BICD2 Monoclonal Antibody (3I3) (Product # MA5-23522) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of BICD2 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a BICD2 Monoclonal Antibody (3I3) (Product # MA5-23522) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of BICD2 was performed in HeLa cells fixed in 4% paraformaldehyde for 10 min. Green: BICD2 Monoclonal Antibody (3I3) (Product # MA5-23522) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of BICD2 was performed in HeLa cells fixed in 4% paraformaldehyde for 10 min. Green: BICD2 Monoclonal Antibody (3I3) (Product # MA5-23522) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Extended Data Fig. 3: SUN1:Nesprin-2G and the dynein adaptor BICD2 mediate nuclear rotation during HCMV infection. a-b, Expression of a SUN1 mutant that does not engage Nesprin-2G impairs nuclear rotation in HCMV-infected cells. a, Representative stills from time lapse recordings of NHDFs expressing Tag-GFP2 forms of SUN1 Full Length (FL) or SUN1 lacking the lumenal domain (SUN1DeltaLu) that mediates interactions with Nesprin-2G, infected with HCMV-UL99mCherry. Rotation traces from this imaging are shown to the right. Analyses focused on cells expressing intermediate levels of SUN1-GFP constructs as high levels of expression can result in retention of Nesprin-2G in the endoplasmic reticulum (ER). b, Quantification of rotation frequencies above or below 180deg; bars represent mean +- SEM, statistics use two-tailed student's t-test, n = 138 cells total from 3 independent biological replicates, ***pLEAA kinesin-binding mutant. e, Western blot analysis of BICD2 expressio
