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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00005660-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005660-M01, RRID:AB_425625
- Product name
- PSAP monoclonal antibody (M01), clone 1D1-C12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant PSAP.
- Antigen sequence
PVLGLKECTRGSAVWCQNVKTASDCGAVKHCLQTV
WNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEIL
VYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIK
GEMSRPGEVCSALNLCESLQKHLAELNHQKQLESN
KIPELDMTEVVAPFMANIPLLLYPQDGPRSKPQPK
DNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVK
EECDRLGPGMADICKNYISQYSEIAIQMMMHMQPK
EICALVGFCDEVKEMPMQTLVPAKVASKNVIPALE
LVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNN
KTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSS
ILSILLEEVSPELVCSMLHLCSGTRLPALTVHVTQ
PKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALE
KGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDP
SFVCLKIGACPSAHKPLLGTEKCIWGPSYWCQNTE
TAAQCNAVEHCKRHVWN- Isotype
- IgG
- Antibody clone number
- 1D1-C12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Serum prosaposin levels are increased in patients with advanced prostate cancer.
Koochekpour S, Hu S, Vellasco-Gonzalez C, Bernardo R, Azabdaftari G, Zhu G, Zhau HE, Chung LW, Vessella RL
The Prostate 2012 Feb;72(3):253-69
The Prostate 2012 Feb;72(3):253-69
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PSAP expression in transfected 293T cell line by PSAP monoclonal antibody (M01), clone 1D1-C12.Lane 1: PSAP transfected lysate(58.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PSAP transfected lysate using anti-PSAP monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PSAP MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to PSAP on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
