HPA004426

antibody from Atlas Antibodies

Targeting: PSAP GLBA, SAP1, SAP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA004426

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA004426, RRID:AB_1079701

Product name

Anti-PSAP

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

TNSTFVQALVEHVKEECDRLGPGMADICKNYISQY
SEIAIQMMMHMQPKEICALVGFCDEVKEMPMQTLV
PAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVC
EFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSL
SEE


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines SK-MEL-30 and HEK293 using Anti-PSAP antibody. Corresponding PSAP RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line SK-MEL-30.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using Anti-PSAP antibody. Corresponding PSAP RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity with a granular pattern in cells of tubules.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN