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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- GTX132295 - Provider product page
- Provider
- GeneTex
- Product name
- Zyxin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Zyxin antibody (GTX132295) diluted at 1:5000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Zyxin knockout (KO) 293T cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Zyxin antibody (GTX132295) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Zyxin antibody detects Zyxin protein at cell junction, cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Zyxin protein stained by Zyxin antibody (GTX132295) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
