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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00007791-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007791-M01, RRID:AB_425750
- Product name
- ZYX monoclonal antibody (M01), clone 2C10-4A7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant ZYX.
- Antigen sequence
MAAPRPSPAISVSVSAPAFYAPQEKFGPVVAPKPK
VNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPED
FPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFP
PAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREK
VSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPP
PVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLP
QVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPV
SLANTQPRGPPASSPAPAPKFSPVTPKFTPVASKF
SPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFT
YAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGP
LTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCG
RCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQ
GQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRM
LRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRP
HCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVA
LDKNFHMKCYECEDCGKPLSIEADDNGCFPLDGHV
LCRKCHTARAQT- Isotype
- IgG
- Antibody clone number
- 2C10-4A7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references NBR1 enables autophagy-dependent focal adhesion turnover.
Mechanical forces facilitate actin polymerization at focal adhesions in a zyxin-dependent manner.
Kenific CM, Stehbens SJ, Goldsmith J, Leidal AM, Faure N, Ye J, Wittmann T, Debnath J
The Journal of cell biology 2016 Feb 29;212(5):577-90
The Journal of cell biology 2016 Feb 29;212(5):577-90
Mechanical forces facilitate actin polymerization at focal adhesions in a zyxin-dependent manner.
Hirata H, Tatsumi H, Sokabe M
Journal of cell science 2008 Sep 1;121(Pt 17):2795-804
Journal of cell science 2008 Sep 1;121(Pt 17):2795-804
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ZYX expression in transfected 293T cell line by ZYX monoclonal antibody (M01), clone 2C10-4A7.Lane 1: ZYX transfected lysate(61.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ZYX is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to ZYX on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of ZYX transfected lysate using anti-ZYX monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ZYX MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
