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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00007791-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007791-M01, RRID:AB_2221180
- Product name
- Mouse Monoclonal Zyxin Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ZYX - zyxin
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Tensile Loads on Tethered Actin Filaments Induce Accumulation of Cell Adhesion-Associated Proteins in Vitro.
Structural Colored Balloon Composed of Temperature-Responsive Polymers Showing LCST Behavior.
NBR1 enables autophagy-dependent focal adhesion turnover.
Hereditary protein S deficiency leads to ischemic stroke.
Mechanical forces facilitate actin polymerization at focal adhesions in a zyxin-dependent manner.
Kiyoshima D, Tatsumi H, Hirata H, Sokabe M
Langmuir : the ACS journal of surfaces and colloids 2019 Jun 11;35(23):7443-7451
Langmuir : the ACS journal of surfaces and colloids 2019 Jun 11;35(23):7443-7451
Structural Colored Balloon Composed of Temperature-Responsive Polymers Showing LCST Behavior.
Higashiguchi K, Morita N, Matsuda K
Langmuir : the ACS journal of surfaces and colloids 2018 Oct 30;34(43):12853-12860
Langmuir : the ACS journal of surfaces and colloids 2018 Oct 30;34(43):12853-12860
NBR1 enables autophagy-dependent focal adhesion turnover.
Kenific CM, Stehbens SJ, Goldsmith J, Leidal AM, Faure N, Ye J, Wittmann T, Debnath J
The Journal of cell biology 2016 Feb 29;212(5):577-90
The Journal of cell biology 2016 Feb 29;212(5):577-90
Hereditary protein S deficiency leads to ischemic stroke.
Wang ZH, Zhao ZJ, Xu K, Sun GB, Song L, Yin HX, Chen XQ
Molecular medicine reports 2015 Sep;12(3):3279-3284
Molecular medicine reports 2015 Sep;12(3):3279-3284
Mechanical forces facilitate actin polymerization at focal adhesions in a zyxin-dependent manner.
Hirata H, Tatsumi H, Sokabe M
Journal of cell science 2008 Sep 1;121(Pt 17):2795-804
Journal of cell science 2008 Sep 1;121(Pt 17):2795-804
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of ZYX over-expressed 293 cell line, cotransfected with ZYX Validated Chimera RNAi ( Cat # H00007791-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZYX monoclonal antibody (M01), clone 2C10-4A7 (Cat # H00007791-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of ZYX expression in transfected 293T cell line by ZYX monoclonal antibody (M01), clone 2C10-4A7.Lane 1: ZYX transfected lysate(61.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Detection limit for recombinant GST tagged ZYX is 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of monoclonal antibody to ZYX on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of ZYX transfected lysate using anti-ZYX monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ZYX MaxPab rabbit polyclonal antibody.
