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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [8]
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- Product number
- PA5-82157 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RhoGAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SEALNQLKLA SIDEKNWPSD EMPDFPKSDD SKSSSPELVT HLKWDDPYYD IARHQIVEVA GDDKYGRKII VFSACRMPPS HQLDHSKLLG YLKHTLDQYV ESDYTLLYLH HGLTSDNKPS LS
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RhoGAP by a RhoGAP polyclonal antibody (Product # PA5-82157). Analysis in human cell line MCF-7.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RhoGAP by a RhoGAP polyclonal antibody (Product # PA5-82157). Analysis in human cell line MCF-7.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RhoGAP by a RhoGAP polyclonal antibody (Product # PA5-82157). Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human Cerebral Cortex tissue.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RhoGAP by a RhoGAP polyclonal antibody (Product # PA5-82157). Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RhoGAP by a RhoGAP polyclonal antibody (Product # PA5-82157). Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RhoGAP in U-251 MG cells using a RhoGAP polyclonal antibody (Product # PA5-82157). The analysis shows localization to vesicles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RhoGAP in mouse dentate gyrus using a RhoGAP polyclonal antibody (Product # PA5-82157). The analysis shows immunoreactivity in polymorph layer.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RhoGAP in human hippocampus using a RhoGAP polyclonal antibody (Product # PA5-82157). The analysis shows positivity in neuropil.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RhoGAP in mouse cingulate cortex using a RhoGAP polyclonal antibody (Product # PA5-82157). The analysis shows cytoplasmic immunoreactivity in neurons.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RhoGAP in mouse thalamus using a RhoGAP polyclonal antibody (Product # PA5-82157). The analysis shows positivity in neurons of the anterodorsal thalamic nucleus.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RhoGAP in mouse motor trigeminal nucleus using a RhoGAP polyclonal antibody (Product # PA5-82157). The analysis shows positivity in neuronal cells bodies and processes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RhoGAP in mouse brain using a RhoGAP polyclonal antibody (Product # PA5-82157). The analysis shows neuronal staining in lateral paragigantocellular nucleus.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RhoGAP in human hippocampus using a RhoGAP polyclonal antibody (Product # PA5-82157). The analysis shows weak immunoreactivity in neuropil.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RhoGAP in human stomach using a RhoGAP polyclonal antibody (Product # PA5-82157). The analysis shows strong cytoplasmic positivity in glandular cells.
