Antibody data
- Antibody Data
- Antigen structure
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- Product number
- A-6473 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NMDAR2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- This antibody recognizes the 180 kDa 2A subunit from rat, mouse and human, and does not cross-react with the 2B or 2C subunits.
Submitted references Adulthood systemic inflammation accelerates the trajectory of age-related cognitive decline.
The NMDA Receptor Subunit (GluN1 and GluN2A) Modulation Following Different Conditions of Cocaine Abstinence in Rat Brain Structures.
Effects of Mephedrone and Amphetamine Exposure during Adolescence on Spatial Memory in Adulthood: Behavioral and Neurochemical Analysis.
Cocaine Self-Administration and Abstinence Modulate NMDA Receptor Subunits and Active Zone Proteins in the Rat Nucleus Accumbens.
LAR receptor phospho-tyrosine phosphatases regulate NMDA-receptor responses.
An activity-dependent local transport regulation via degradation and synthesis of KIF17 underlying cognitive flexibility.
Development and Characterization of Human Cerebral Organoids: An Optimized Protocol.
Homocysteine sensitizes the mouse neuromuscular junction to oxidative stress by nitric oxide.
Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction.
Mechanisms of Functional Hypoconnectivity in the Medial Prefrontal Cortex of Mecp2 Null Mice.
SALM4 suppresses excitatory synapse development by cis-inhibiting trans-synaptic SALM3-LAR adhesion.
Chronic copper exposure causes spatial memory impairment, selective loss of hippocampal synaptic proteins, and activation of PKR/eIF2α pathway in mice.
PSD95 suppresses dendritic arbor development in mature hippocampal neurons by occluding the clustering of NR2B-NMDA receptors.
Antibody-mediated targeted gene transfer of helper virus-free HSV-1 vectors to rat neocortical neurons that contain either NMDA receptor 2B or 2A subunits.
Estradiol potentiation of NR2B-dependent EPSCs is not due to changes in NR2B protein expression or phosphorylation.
Cellular and synaptic mechanisms of anti-NMDA receptor encephalitis.
Neto1 is a novel CUB-domain NMDA receptor-interacting protein required for synaptic plasticity and learning.
Neuronal interleukin-16 (NIL-16): a dual function PDZ domain protein.
Neuronal interleukin-16 (NIL-16): a dual function PDZ domain protein.
Barter J, Kumar A, Bean L, Ciesla M, Foster TC
Aging 2021 Sep 29;13(18):22092-22108
Aging 2021 Sep 29;13(18):22092-22108
The NMDA Receptor Subunit (GluN1 and GluN2A) Modulation Following Different Conditions of Cocaine Abstinence in Rat Brain Structures.
Smaga I, Wydra K, Suder A, Frankowska M, Sanak M, Caffino L, Fumagalli F, Filip M
Neurotoxicity research 2021 Jun;39(3):556-565
Neurotoxicity research 2021 Jun;39(3):556-565
Effects of Mephedrone and Amphetamine Exposure during Adolescence on Spatial Memory in Adulthood: Behavioral and Neurochemical Analysis.
Grochecki P, Smaga I, Lopatynska-Mazurek M, Gibula-Tarlowska E, Kedzierska E, Listos J, Talarek S, Marszalek-Grabska M, Hubalewska-Mazgaj M, Korga-Plewko A, Dudka J, Marzec Z, Filip M, Kotlinska JH
International journal of molecular sciences 2021 Jan 8;22(2)
International journal of molecular sciences 2021 Jan 8;22(2)
Cocaine Self-Administration and Abstinence Modulate NMDA Receptor Subunits and Active Zone Proteins in the Rat Nucleus Accumbens.
Smaga I, Wydra K, Frankowska M, Fumagalli F, Sanak M, Filip M
Molecules (Basel, Switzerland) 2020 Jul 31;25(15)
Molecules (Basel, Switzerland) 2020 Jul 31;25(15)
LAR receptor phospho-tyrosine phosphatases regulate NMDA-receptor responses.
Sclip A, Südhof TC
eLife 2020 Jan 27;9
eLife 2020 Jan 27;9
An activity-dependent local transport regulation via degradation and synthesis of KIF17 underlying cognitive flexibility.
Iwata S, Morikawa M, Takei Y, Hirokawa N
Science advances 2020 Dec;6(51)
Science advances 2020 Dec;6(51)
Development and Characterization of Human Cerebral Organoids: An Optimized Protocol.
Yakoub AM, Sadek M
Cell transplantation 2018 Mar;27(3):393-406
Cell transplantation 2018 Mar;27(3):393-406
Homocysteine sensitizes the mouse neuromuscular junction to oxidative stress by nitric oxide.
Wang JS, Bojovic D, Chen Y, Lindgren CA
Neuroreport 2018 Aug 15;29(12):1030-1035
Neuroreport 2018 Aug 15;29(12):1030-1035
Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction.
Lisek M, Ferenc B, Studzian M, Pulaski L, Guo F, Zylinska L, Boczek T
Frontiers in cellular neuroscience 2017;11:181
Frontiers in cellular neuroscience 2017;11:181
Mechanisms of Functional Hypoconnectivity in the Medial Prefrontal Cortex of Mecp2 Null Mice.
Sceniak MP, Lang M, Enomoto AC, James Howell C, Hermes DJ, Katz DM
Cerebral cortex (New York, N.Y. : 1991) 2016 May;26(5):1938-1956
Cerebral cortex (New York, N.Y. : 1991) 2016 May;26(5):1938-1956
SALM4 suppresses excitatory synapse development by cis-inhibiting trans-synaptic SALM3-LAR adhesion.
Lie E, Ko JS, Choi SY, Roh JD, Cho YS, Noh R, Kim D, Li Y, Kang H, Choi TY, Nam J, Mah W, Lee D, Lee SG, Kim HM, Kim H, Choi SY, Um JW, Kang MG, Bae YC, Ko J, Kim E
Nature communications 2016 Aug 2;7:12328
Nature communications 2016 Aug 2;7:12328
Chronic copper exposure causes spatial memory impairment, selective loss of hippocampal synaptic proteins, and activation of PKR/eIF2α pathway in mice.
Ma Q, Ying M, Sui X, Zhang H, Huang H, Yang L, Huang X, Zhuang Z, Liu J, Yang X
Journal of Alzheimer's disease : JAD 2015;43(4):1413-27
Journal of Alzheimer's disease : JAD 2015;43(4):1413-27
PSD95 suppresses dendritic arbor development in mature hippocampal neurons by occluding the clustering of NR2B-NMDA receptors.
Bustos FJ, Varela-Nallar L, Campos M, Henriquez B, Phillips M, Opazo C, Aguayo LG, Montecino M, Constantine-Paton M, Inestrosa NC, van Zundert B
PloS one 2014;9(4):e94037
PloS one 2014;9(4):e94037
Antibody-mediated targeted gene transfer of helper virus-free HSV-1 vectors to rat neocortical neurons that contain either NMDA receptor 2B or 2A subunits.
Cao H, Zhang GR, Geller AI
Brain research 2011 Sep 30;1415:127-35
Brain research 2011 Sep 30;1415:127-35
Estradiol potentiation of NR2B-dependent EPSCs is not due to changes in NR2B protein expression or phosphorylation.
Snyder MA, Cooke BM, Woolley CS
Hippocampus 2011 Apr;21(4):398-408
Hippocampus 2011 Apr;21(4):398-408
Cellular and synaptic mechanisms of anti-NMDA receptor encephalitis.
Hughes EG, Peng X, Gleichman AJ, Lai M, Zhou L, Tsou R, Parsons TD, Lynch DR, Dalmau J, Balice-Gordon RJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Apr 28;30(17):5866-75
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Apr 28;30(17):5866-75
Neto1 is a novel CUB-domain NMDA receptor-interacting protein required for synaptic plasticity and learning.
Ng D, Pitcher GM, Szilard RK, Sertié A, Kanisek M, Clapcote SJ, Lipina T, Kalia LV, Joo D, McKerlie C, Cortez M, Roder JC, Salter MW, McInnes RR
PLoS biology 2009 Feb 24;7(2):e41
PLoS biology 2009 Feb 24;7(2):e41
Neuronal interleukin-16 (NIL-16): a dual function PDZ domain protein.
Kurschner C, Yuzaki M
The Journal of neuroscience : the official journal of the Society for Neuroscience 1999 Sep 15;19(18):7770-80
The Journal of neuroscience : the official journal of the Society for Neuroscience 1999 Sep 15;19(18):7770-80
Neuronal interleukin-16 (NIL-16): a dual function PDZ domain protein.
Kurschner C, Yuzaki M
The Journal of neuroscience : the official journal of the Society for Neuroscience 1999 Sep 15;19(18):7770-80
The Journal of neuroscience : the official journal of the Society for Neuroscience 1999 Sep 15;19(18):7770-80
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Supportive validation
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- Immunohistochemistry using NMDA Receptor Subunit 2A Polyclonal Antibody, Rabbit: Rat brain cryosections labeled with anti-NMDA receptor, subunit 2A (rat), rabbit IgG fraction (Product # A-6473) and detected using Alexa Fluor® 488 goat anti-rabbit IgG antibody (Product # A-11008). The tissue was also labeled with Alexa Fluor® 594 anti-glial fibrillary acidic protein antibody (Product # A-21295) and counterstained with TOTO®-3 iodide (Product # T3604), which was pseudocolored light blue in this image.
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- Immunohistochemistry using NMDA Receptor Subunit 2A Polyclonal Antibody, Rabbit: Rat brain cryosections labeled with anti-NMDA receptor, subunit 2A (rat) antibody, rabbit IgG fraction (Product # A-6473) and detected using Alexa Fluor 488 goat anti-rabbit IgG antibody (Product # A-11008). The tissue was also labeled with Alexa Fluor 594 anti-glial fibrillary acidic protein antibody (Product # A-21295) and counterstained with TOTO-3 iodide (T3604), which was pseudocolored light blue in this image.
Supportive validation
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- Figure 3 Expression of NMDAR subunits NR2A and NR2B, and MAGUKs PSD95 and SAP102, at different stages of development. A , Total protein extracts were obtained from hippocampal neurons at 2, 5, 7, 12, 15, 18, and 20 DIV and immunoblotted with antibodies against NR2B, NR2A, PSD95, SAP102, or N-cadherin as loading control. B , Results of qRT-PCR run with primers designed to determine mRNA levels for NR2B, NR2A, PSD95, or SAP102 at the same developmental stages; results were normalized against levels of GAPDH mRNA. Note that NR2B expression decreases with development, while NR2A and PSD95 increases. Expression of SAP102 remains relatively stable at these developmental stages. C , Immunodetection of endogenous NR2B, NR2A, PSD95, or SAP102 (green) and co-localization with presynaptic marker Bassoon or Piccolo (red) at 5 and 20 DIV. Insets show magnified views of boxed areas showing examples of synaptic clusters located in close apposition to presynaptic clusters.
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- Figure 6 Interaction of PSD95 with NMDAR subunits and PMCA isoforms. Brain lysates were incubated with anti-PSD95 antibodies conjugated to sepharose beads. Immunocomplexes were recovered and resolved by SDS-PAGE and the membranes were next probed with anti-NMDAR1 (A) , anti-NMDAR2A (B) , anti-NMDAR2B (C) , 5F10 (D) , anti-PMCA2 (E) or anti-PMCA4 (F) antibodies. Negative controls included sepharose-linked secondary antibodies (Control Ab) or sepharose beads only. PSD95 was also immunoprobed in beads-unbound fraction (unbound). Representative blots are shown. The bands intensity were densitometically quantified and the results were expressed as AU defined as the optical density per mg of protein (OD/mg protein). * P < 0.05 ketamine treated vs. saline, n = 5. CX, cortex; CB, cerebellum; H, hippocampus; ST, striatum.
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- Figure 5 Western blot analysis of PSD95 and N-methyl-D-aspartic acid receptor (NMDAR) subunit protein level in synaptosomal membranes. The protein level of PSD95, NMDAR1, NMDAR2A and NMDAR2B was determined by immunoblotting (A) and the bands intensity corresponding to the target protein was densitometrically analyzed (B) . The results are expressed as AU obtained following normalization to endogenous Na + /K + -ATPase level. Representative blots are shown. * P < 0.05 ketamine treated vs. saline, n = 5. CX, cortex; CB, cerebellum; H, hippocampus; ST, striatum.
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- Figure 4 Neto1 Binds to NMDA Receptors Independently of the C-Terminal PDZ Ligand (A, B) Immunoblots of immunoprecipitates from transfected HEK293 cell lysates. The transfected cDNAs are shown above each lane. CSF-1 EC-eGFP encodes the extracellular domain of CSF-1 fused to eGFP. Nrpn1 CUB12-eGFP encodes the two CUB domains from neuropilin-1 fused to eGFP. Blot, antibody used for immunoblot analysis; IP, antibody used for immunoprecipitation.
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- Fig. 6 Newly synthesized mEos3.2-KIF17 moves along the dendrites and colocalizes with NR2B. ( A to D ) Time-lapse images of newly synthesized mEos3.2-KIF17(+) (A), mEos3.2-KIF17(DeltaM) (B), and mEos3.2-KIF17(DeltaT) (C) along dendrites and their kymographs (D). Hippocampal neurons (DIV 17 to 19) transfected with mEos3.2-KIF17(+)3'UTR, mEos3.2-KIF17(DeltaM)3'UTR, and mEos3.2-KIF17(DeltaT)3'UTR were stimulated with NMDA (20 muM) after photoconversion of mEos3.2. Yellow arrowheads in (A) to (C), newly synthesized mEos3.2 fused to each construct of KIF17. Black arrowheads in (D), the time and location of KIF17 3'UTR-mediated local synthesis. Red lines in (A) to (C) trace the morphology of the dendrites. Scale bars, 5 mum. See also fig. S8. ( E and F ) Quantifications as average time when mEos3.2 fused to each construct of KIF17 was synthesized from NMDA bath application (E) and velocities for movements of mEos3.2 fused to each construct of KIF17 (F). P >= 0.05 (E), P < 0.001 (F), one-way ANOVA; *** P < 0.001, Bonferroni's post hoc comparison (F). n = 7 moving puncta from six culture neurons. ( G to I ) Representative images of dendrites expressing mEos3.2-KIF17(+) or mEos3.2-KIF17(DeltaT), which were immunostained with anti-NR2B or anti-NR2A antibodies (G), and comparisons of the colocalization of the anti-NR2B-positive puncta (H) and that of mEos3.2-KIF17(+) (I) in dendrites. Scale bar, 5 mum. *** P < 0.001, two-tailed t test. n = 7 dendrites from seven culture neurons.
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- Figure 4 Effect of repeated mephedrone or amphetamine administration during adolescence on MMP-9 ( A ) and NMDA subunits (GluN1, GluN2A, GluN2B) and PSD-95 ( B ) expression in the prefrontal cortex (PC) and hippocampus (HC) of rats (PND 87) that underwent the Barnes maze task. Representative blots for significant changes are presented, as well as full membranes are presented in the supplementary material (Figure S1) . Data are expressed as mean +- SEM. n = 8 rats/group. * p < 0.05 vs vehicle (0.9% NaCl). V--vehicle, M--mephedrone, A--amphetamine.
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- Figure 3 Changes in the expression of NMDA receptor subunits (GluN1, GluN2A and GluN2B) in the nucleus accumbens in rats following cocaine self-administration. All data are expressed as % of control (mean +- SEM). N = 6 rats/group. * p < 0.05 vs. yoked saline. ( a ), GluN1 protein levels [% of control]; ( b ), GluN2A protein levels [% of control]. ; ( c ), GluN2B protein levels [% of control].
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- Fig. 3 Changes in the expression of GluN2A subunit in the PSD fraction of PLC and dHIP in rats following cocaine abstinence with extinction training a . Full membranes for GluN2A subunit are presented b . PLC-prelimbic cortex, dHIP-dorsal hippocampus, PSD-postsynaptic density, YS-yoked saline, YC-yoked cocaine, SA-cocaine self-administration. All data are expressed as mean +- SEM. N = 8 rats/group. * p < 0.05 vs. yoked saline
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- Figure 5 Western blot analysis of NMDAR subunit expression in CA1 region of hippocampus. The blots illustrate expression of ( A ) GluN2B, ( B ) GluN2A, and PSD95. ( C ) The bars represent the mean (+- SEM) ratio of expression for vehicle ( n = 4) and LPS treated ( n = 4) animals.