PA5-35377

antibody from Invitrogen Antibodies

Targeting: GRIN2A GluN2A, NMDAR2A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35377

Provider

Invitrogen Antibodies

Product name

NMDAR2A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

PA5-35377 will not cross-react with NR2B and NR2C subunits.

Concentration

~0.2 mg/mL

References

Submitted references

A novel bio-inspired strategy to prevent amyloidogenesis and synaptic damage in Alzheimer's disease.

Catania M, Colombo L, Sorrentino S, Cagnotto A, Lucchetti J, Barbagallo MC, Vannetiello I, Vecchi ER, Favagrossa M, Costanza M, Giaccone G, Salmona M, Tagliavini F, Di Fede G
Molecular psychiatry 2022 Dec;27(12):5227-5234

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Timing-dependent LTP and LTD in mouse primary visual cortex following different visual deprivation models.

Guo Y, Zhang W, Chen X, Fu J, Cheng W, Song D, Qu X, Yang Z, Zhao K
PloS one 2017;12(5):e0176603

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SorCS2-mediated NR2A trafficking regulates motor deficits in Huntington's disease.

Ma Q, Yang J, Milner TA, Vonsattel JG, Palko ME, Tessarollo L, Hempstead BL
JCI insight 2017 May 4;2(9)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NMDA Receptor 2A in rat hippocampal lysate (10 µg) using a NMDA Receptor 2A polyclonal antibody (Product # PA5-35377). Results show a band at ~180 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of NMDAR2A in rat hippocampal lysate (10 µg), showing specific immunolabeling of a band at ~180 kDa corresponding to the NR2A subunit of the NMDAR2A polyclonal antibody (Product # PA5-35377) receptor.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NMDAR2A in rabbit retina showing NMDAR2A polyclonal antibody (Product # PA5-35377) in the rod and cone photoreceptors in the outer plexiform layer as well as the entire inner plexiform layer.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 3 DE and MD modify NMDAR subunit composition in synaptoneurosomes from mice visual cortex. (A-B) top: Representative immunoblots of synaptoneurosome samples prepared from frontal (F) and visual (V) cortices of mice that were normal-reared (NR), dark-exposured (DE) and monocular deprived mice. Blots were probed with subunit-specific antibodies for NR2A (Fig A), NR2B(Fig B). Bottom: Summary of immunoblot analysis of synaptoneurosome samples prepared from Visual cortices (V) of NR and DE mice or NR and MD mice. NR2A protein levels in visual cortex weresignificantly reduced after 6 days of DE ( t -test p0.6)(Fig B). Data are expressed as mean values +- SEM normalized to the average NR protein level, n = 8 from 4 animals for each group. (C) The NR2A/2B ratio is reduced in the deprived visual cortex from mice that were under dark exposure (t -test p