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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-82174 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HGS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LAEDIDPELA RYLNRNYWEK KQEEARKSPT PSAPVPLTEP AAQPGEGHAA PTNVVENPLP ETDSQPIPPS GGPFSEPQFH NGESEESHEQ FLKALQNAVT TFVNRMKSNH MRGRSITNDS
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HGS by a HGS polyclonal antibody (Product # PA5-82174). Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401488).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HGS in U-2 OS cells using a HGS polyclonal antibody (Product # PA5-82174). The analysis shows localization to cytosol & vesicles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HGS in human testis using a HGS polyclonal antibody (Product # PA5-82174). The analysis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HGS in human cerebral cortex using a HGS polyclonal antibody (Product # PA5-82174). The analysis shows moderate positivity in neuropil.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HGS in human tonsil using a HGS polyclonal antibody (Product # PA5-82174). The analysis shows moderate cytoplasmic positivity in germinal center cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HGS in human skeletal muscle using a HGS polyclonal antibody (Product # PA5-82174). The analysis shows no positivity in myocytes as expected.
