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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- R32346 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Daxx Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids 56-345 of human Daxx were used as the immunogen for the Daxx antibody.
- Description
- Antigen affinity
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the Daxx antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat testis, 2) human A431, 3) HeLa, 4) HUT and 5) mouse HEPA lysate with Daxx antibody. Predicted molecular weight ~81 kDa but routinely observed at ~120 kDa, the slow SDS-PAGE migration possibly due to the proteins high acidic residue content.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of human SMMC-7721 cells with Daxx antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of human A549 cells with Daxx antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human intestinal cancer tissue with Daxx antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat intestine with Daxx antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
