Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-85309 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85309, RRID:AB_11046575
- Product name
- Rabbit Polyclonal Daxx Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Daxx antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references TERT promoter mutations are frequent and show association with MED12 mutations in phyllodes tumors of the breast.
Epigenetic dysregulation and poorer prognosis in DAXX-deficient pancreatic neuroendocrine tumours.
ATRX represses alternative lengthening of telomeres.
Whole-exome sequencing identifies somatic ATRX mutations in pheochromocytomas and paragangliomas.
Sp100 provides intrinsic immunity against human papillomavirus infection.
Loss of ATRX or DAXX expression and concomitant acquisition of the alternative lengthening of telomeres phenotype are late events in a small subset of MEN-1 syndrome pancreatic neuroendocrine tumors.
Driver mutations in histone H3.3 and chromatin remodelling genes in paediatric glioblastoma.
Altered telomeres in tumors with ATRX and DAXX mutations.
Yoshida M, Ogawa R, Yoshida H, Maeshima A, Kanai Y, Kinoshita T, Hiraoka N, Sekine S
British journal of cancer 2015 Oct 20;113(8):1244-8
British journal of cancer 2015 Oct 20;113(8):1244-8
Epigenetic dysregulation and poorer prognosis in DAXX-deficient pancreatic neuroendocrine tumours.
Pipinikas CP, Dibra H, Karpathakis A, Feber A, Novelli M, Oukrif D, Fusai G, Valente R, Caplin M, Meyer T, Teschendorff A, Bell C, Morris TJ, Salomoni P, Luong TV, Davidson B, Beck S, Thirlwell C
Endocrine-related cancer 2015 Jun;22(3):L13-8
Endocrine-related cancer 2015 Jun;22(3):L13-8
ATRX represses alternative lengthening of telomeres.
Napier CE, Huschtscha LI, Harvey A, Bower K, Noble JR, Hendrickson EA, Reddel RR
Oncotarget 2015 Jun 30;6(18):16543-58
Oncotarget 2015 Jun 30;6(18):16543-58
Whole-exome sequencing identifies somatic ATRX mutations in pheochromocytomas and paragangliomas.
Fishbein L, Khare S, Wubbenhorst B, DeSloover D, D'Andrea K, Merrill S, Cho NW, Greenberg RA, Else T, Montone K, LiVolsi V, Fraker D, Daber R, Cohen DL, Nathanson KL
Nature communications 2015 Jan 21;6:6140
Nature communications 2015 Jan 21;6:6140
Sp100 provides intrinsic immunity against human papillomavirus infection.
Stepp WH, Meyers JM, McBride AA
mBio 2013 Nov 5;4(6):e00845-13
mBio 2013 Nov 5;4(6):e00845-13
Loss of ATRX or DAXX expression and concomitant acquisition of the alternative lengthening of telomeres phenotype are late events in a small subset of MEN-1 syndrome pancreatic neuroendocrine tumors.
de Wilde RF, Heaphy CM, Maitra A, Meeker AK, Edil BH, Wolfgang CL, Ellison TA, Schulick RD, Molenaar IQ, Valk GD, Vriens MR, Borel Rinkes IH, Offerhaus GJ, Hruban RH, Matsukuma KE
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2012 Jul;25(7):1033-9
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2012 Jul;25(7):1033-9
Driver mutations in histone H3.3 and chromatin remodelling genes in paediatric glioblastoma.
Schwartzentruber J, Korshunov A, Liu XY, Jones DT, Pfaff E, Jacob K, Sturm D, Fontebasso AM, Quang DA, Tönjes M, Hovestadt V, Albrecht S, Kool M, Nantel A, Konermann C, Lindroth A, Jäger N, Rausch T, Ryzhova M, Korbel JO, Hielscher T, Hauser P, Garami M, Klekner A, Bognar L, Ebinger M, Schuhmann MU, Scheurlen W, Pekrun A, Frühwald MC, Roggendorf W, Kramm C, Dürken M, Atkinson J, Lepage P, Montpetit A, Zakrzewska M, Zakrzewski K, Liberski PP, Dong Z, Siegel P, Kulozik AE, Zapatka M, Guha A, Malkin D, Felsberg J, Reifenberger G, von Deimling A, Ichimura K, Collins VP, Witt H, Milde T, Witt O, Zhang C, Castelo-Branco P, Lichter P, Faury D, Tabori U, Plass C, Majewski J, Pfister SM, Jabado N
Nature 2012 Jan 29;482(7384):226-31
Nature 2012 Jan 29;482(7384):226-31
Altered telomeres in tumors with ATRX and DAXX mutations.
Heaphy CM, de Wilde RF, Jiao Y, Klein AP, Edil BH, Shi C, Bettegowda C, Rodriguez FJ, Eberhart CG, Hebbar S, Offerhaus GJ, McLendon R, Rasheed BA, He Y, Yan H, Bigner DD, Oba-Shinjo SM, Marie SK, Riggins GJ, Kinzler KW, Vogelstein B, Hruban RH, Maitra A, Papadopoulos N, Meeker AK
Science (New York, N.Y.) 2011 Jul 22;333(6041):425
Science (New York, N.Y.) 2011 Jul 22;333(6041):425
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Daxx Antibody [NBP1-85309] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-DAXX antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Daxx Antibody [NBP1-85309] - Analysis in control (vector only transfected HEK293T lysate) and DAXX over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts and in Leydig cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309] - Staining of human tonsil shows moderate to strong nuclear positivity in lymphoid cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Daxx Antibody [NBP1-85309] - Staining in human testis and skeletal muscle tissues . Corresponding DAXX RNA-seq data are presented for the same tissues.