MA5-15552

antibody from Invitrogen Antibodies

Targeting: DAXX DAP6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15552

Provider

Invitrogen Antibodies

Product name

DAXX Monoclonal Antibody (7A11)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15552 targets DAXX in FACS, IF, and WB applications and shows reactivity with Human samples.

Antibody clone number

7A11

Concentration

1.0 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DAXX using DAXX monoclonal antibody (Product # MA5-15552) in K562 cell lysate (1).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-DAXX Monoclonal Antibody (7A11) (Product # MA5-15552) and a 100kDa band corresponding to DAXX was observed in all cell lysates tested. Whole cell lysates (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), K-562 (Lane 3), MCF7 (Lane 4), U-2 OS (Lane 5) and SH-SY5Y (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PANC-1 cells using DAXX monoclonal antibody (Product # MA5-15552) (Green). Blue: DRAQ5 fluorescent DNA dye.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using DAXX monoclonal antibody (Product # MA5-15552) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using DAXX monoclonal antibody (Product # MA5-15552) (green) and negative control (purple).