PA5-20570

antibody from Invitrogen Antibodies

Targeting: CERS5 FLJ25304, LASS5, MGC45411, Trh4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20570

Provider

Invitrogen Antibodies

Product name

Lass5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is rat brain tissue lysate.

Concentration

1 mg/mL

References

Submitted references

The glucocorticoid-Angptl4-ceramide axis induces insulin resistance through PP2A and PKCζ.

Chen TC, Benjamin DI, Kuo T, Lee RA, Li ML, Mar DJ, Costello DE, Nomura DK, Wang JC
Science signaling 2017 Jul 25;10(489)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain tissue lysate using a LASS5 polyclonal antibody (Product # PA5-20570) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of LASS5 in rat brain tissue lysate with Lass5 Polyclonal Antibody (Product # PA5-20570) at (A) 1 and (B) 2 µg/mL.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of human brain cells using a LASS5 polyclonal antibody (Product # PA5-20570) at a 20 µg/mL dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of LASS5 in Human Brain cells with Lass5 Polyclonal Antibody (Product # PA5-20570) at 20 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of LASS5 in Human Brain cells with Lass5 Polyclonal Antibody (Product # PA5-20570) at 20 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of LASS5 in human brain tissue with Lass5 Polyclonal Antibody (Product # PA5-20570) at 2.5 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4. Dexamethasone-activated hepatic ceramide synthetic program was attenuated in Angptl4 -/- mice. WT and Angptl4 -/- mice were treated with PBS or dexamethasone for 7 days. ( A to C ) The concentrations of 16 different ceramide species in the liver (A), 5 different free fatty acids (FFAs) in plasma (B), and 4 ceramide species in plasma (C) of these mice were measured. n = 4 mice per group. ( D ) The expression of genes encoding enzymes involved in ceramide production was monitored using quantitative polymerase chain reaction (qPCR). The heat map shows the relative expression compared to that in the WT PBS group. Red shading on the heat map indicates higher expression, and green shading represents lower expression. The changes in fold induction are shown in fig. S1 . n = 16 mice per group. ( E ) Schematic representation of ceramide synthesis pathways. The genes and metabolites that were induced by dexamethasone in WT mice liver are shown in red. The metabolites that were reduced by dexamethasone are shown in green. ( F ) The abundance of Cers5 and Cers6 proteins was monitored using Western blot. The bar graph represents the average intensity of bands normalized to those for Gapdh. Error bars represent SEM. n = 3 to 4 mice per group. * P < 0.05.