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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ABIN500138 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-LAG1 Homolog, Ceramide Synthase 5 (LASS5) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- LASS5 antibody was raised against a 17 amino acid peptide near the carboxy terminus of the human LASS5.
- Description
- Peptide affinity chromatography
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Epitope
- C-Term
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 2 - 8°C for up to one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Necessary role for the Lag1p motif in (dihydro)ceramide synthase activity.
LASS5 is a bona fide dihydroceramide synthase that selectively utilizes palmitoyl-CoA as acyl donor.
LASS5 is the predominant ceramide synthase isoform involved in de novo sphingolipid synthesis in lung epithelia.
Two mammalian longevity assurance gene (LAG1) family members, trh1 and trh4, regulate dihydroceramide synthesis using different fatty acyl-CoA donors.
Spassieva S, Seo JG, Jiang JC, Bielawski J, Alvarez-Vasquez F, Jazwinski SM, Hannun YA, Obeid LM
The Journal of biological chemistry 2006 Nov 10;281(45):33931-8
The Journal of biological chemistry 2006 Nov 10;281(45):33931-8
LASS5 is a bona fide dihydroceramide synthase that selectively utilizes palmitoyl-CoA as acyl donor.
Lahiri S, Futerman AH
The Journal of biological chemistry 2005 Oct 7;280(40):33735-8
The Journal of biological chemistry 2005 Oct 7;280(40):33735-8
LASS5 is the predominant ceramide synthase isoform involved in de novo sphingolipid synthesis in lung epithelia.
Xu Z, Zhou J, McCoy DM, Mallampalli RK
Journal of lipid research 2005 Jun;46(6):1229-38
Journal of lipid research 2005 Jun;46(6):1229-38
Two mammalian longevity assurance gene (LAG1) family members, trh1 and trh4, regulate dihydroceramide synthesis using different fatty acyl-CoA donors.
Riebeling C, Allegood JC, Wang E, Merrill AH Jr, Futerman AH
The Journal of biological chemistry 2003 Oct 31;278(44):43452-9
The Journal of biological chemistry 2003 Oct 31;278(44):43452-9
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of LASS5 in SK-N-SH lysate with AP30490PU-N at 1 ?g/ml in the (A) absence and (B) presence of blocking peptide.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunofluorescence of LASS5 in human brain tissue with AP30490PU-N at 20 ?g/ml.
